Team:University College London/Module 2/Results

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Based on the results we have obtained, we have determined that the biofilms produced by cells transformed with BBa_K729018 have a two-fold shear resistance as compared to the biofilms of the control W3110.
Based on the results we have obtained, we have determined that the biofilms produced by cells transformed with BBa_K729018 have a two-fold shear resistance as compared to the biofilms of the control W3110.
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[[File:UniversityCollegeLondon_Curli_Shear_Stress.png|500px|centre]]
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[[File:UniversityCollegeLondon_Curli_Shear_Stress.png|450px|centre]]

Revision as of 15:17, 26 September 2012

Module 2: Aggregation

Description | Design | Construction | Characterisation | Shear Device | Modelling | Results | Conclusions

Congo Red Agar Assay

We have used a congo red agar assay to determine if our curli BioBrick transformed cells have successfully expressed the amyloid fibrins. Congo red is a diazo dye that binds to the curli fibrins, thereby causing cells positive for curlis to appear red when grown on this agar.

As can be seen from plates below, our cells transformed with BBa_K729017 display a red tinge to their colonies, while the untransformed W3110 cells do not. We hence conclude that we have successfully transformed our cells to produce curlis.

Congo Red Agar Assay
Control BBa_K729017
UniversityCollegeLondon Curli Congo Red Control.jpg UniversityCollegeLondon Curli Congo Red K729017.jpg

Shear Force Assay

Using a shear device...

Based on the results we have obtained, we have determined that the biofilms produced by cells transformed with BBa_K729018 have a two-fold shear resistance as compared to the biofilms of the control W3110.

UniversityCollegeLondon Curli Shear Stress.png