Team:University College London/Module 2/Design

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== Design ==
== Design ==
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Module 2 was designed to meet the following requirements
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<html><div align="center"><img src="http://2012.igem.org/wiki/images/2/2a/UclCurli.png" alt="Aggregation" /></div></html>
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'''Requirement 1) Cells must express an adhesive element that allow them to bind to plastics.'''
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Module 2 was designed to meet the following requirements:
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'''Requirement 1: Cells must express an adhesive element that allow them to bind to plastics.'''
Curlis are adherent proteins that allow biofilm  <span class="footnote" title="Curlinonspec">formation</span>. From the reference papers, curlis expressed bind more readily to plastics at 30˚C than 37˚C, a factor that would be ideal if the system were to be applied to the marine environment.
Curlis are adherent proteins that allow biofilm  <span class="footnote" title="Curlinonspec">formation</span>. From the reference papers, curlis expressed bind more readily to plastics at 30˚C than 37˚C, a factor that would be ideal if the system were to be applied to the marine environment.
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'''Requirement 2) The adhesion system must be compatible with the chassis being used.'''
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'''Requirement 2: The adhesion system must be compatible with the chassis being used.'''
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Curlis are native to E.Coli, and have been previously deliberately expressed (Chirwa et al. 2003). Furthermore, a curli BioBrick already exist in the Parts Registry (K540000), albeit with a cobalt promoter that will be replaced for the purposes of the project. As curlis have not been expressed before in Roseobacter, this is something we wish to investigate.
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Curlis are native to ''E. coli'', and have been previously deliberately <span class="footnote" title="chirwa">formation</span> expressed. Furthermore, a curli BioBrick already exist in the Parts Registry (BBa_K540000), albeit with a cobalt promoter that will be replaced for the purposes of the project. As curlis have not been expressed before in Roseobacter, this is something we wish to investigate.
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'''Requirement 3) The adhesion system must be compatible with the real world environment that the cells would be expected to be exposed to.'''
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'''Requirement 3: The adhesion system must be compatible with the real world environment that the cells would be expected to be exposed to.'''
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Curlis express favourable at temperatures from 26-30˚C, and during the cellular stationary growth phase (Szabó et al. 2005). These conditions correspond well to the marine environment that we hope to target in our project.Two important unknowns also exist that we wish to characterise. Firstly, Curli expression is abrogated in bacteria cultured in the presence of salt, and we want to see if our system can work effectively at the salt levels found in the ocean. Secondly, we need to see whether our system will be strong enough to endure the shear stress of the ocean. When our characterisation plans are on the wiki, you will be able to read more about this aspect of our experiments.
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Curlis express favourable at temperatures from 26-30˚C, and during the cellular stationary growth <span class="footnote" title="Curlinonspec">phase</span>. These conditions correspond well to the marine environment that we hope to target in our project. Two important unknowns also exist that we wish to characterise. Firstly, curli expression is abrogated in bacteria cultured in the presence of salt, and we want to see if our system can work effectively at the salt levels found in the ocean. Secondly, we need to see whether our system will be strong enough to endure the shear stress of the ocean.

Latest revision as of 00:17, 27 September 2012

Module 2: Aggregation

Description | Design | Construction | Characterisation | Shear Device | Modelling | Results | Conclusions

Design

Aggregation


Module 2 was designed to meet the following requirements:


Requirement 1: Cells must express an adhesive element that allow them to bind to plastics.

Curlis are adherent proteins that allow biofilm formation. From the reference papers, curlis expressed bind more readily to plastics at 30˚C than 37˚C, a factor that would be ideal if the system were to be applied to the marine environment.


Requirement 2: The adhesion system must be compatible with the chassis being used.

Curlis are native to E. coli, and have been previously deliberately formation expressed. Furthermore, a curli BioBrick already exist in the Parts Registry (BBa_K540000), albeit with a cobalt promoter that will be replaced for the purposes of the project. As curlis have not been expressed before in Roseobacter, this is something we wish to investigate.


Requirement 3: The adhesion system must be compatible with the real world environment that the cells would be expected to be exposed to.

Curlis express favourable at temperatures from 26-30˚C, and during the cellular stationary growth phase. These conditions correspond well to the marine environment that we hope to target in our project. Two important unknowns also exist that we wish to characterise. Firstly, curli expression is abrogated in bacteria cultured in the presence of salt, and we want to see if our system can work effectively at the salt levels found in the ocean. Secondly, we need to see whether our system will be strong enough to endure the shear stress of the ocean.