Team:University College London/LabBook/Week3

Monday (18.6.12)

Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.

In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.

Method

(LOGO) – Day 1 of Generating Competent Cells

Tuesday (19.6.12)

Aim – Picking Colonies: Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation

Method

(LOGO) – Day 2 of Generating Competent Cells

Wednesday (20.6.12)

Aim – Permeabilisation: Incubating Cells in the presences of CaCl2 will prepare the cell wall, such that it becomes permeable to DNA.

Method

(LOGO) – Day 3 of Generating Competent Cells