Team:University College London/LabBook/Week3
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- | == | + | ==Monday (18.6.12)== |
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+ | Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar. | ||
+ | |||
+ | Method | ||
+ | |||
+ | (LOGO) – Day 1 of Generating Competent Cells | ||
+ | |||
+ | Tuesday (19.6.12) | ||
+ | |||
+ | Aim – Day 2 of Generating Competent Cells: Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation | ||
+ | |||
+ | (LOGO) – Day 2 of Generating Competent Cells | ||
+ | |||
+ | Wednesday (20.6.12) | ||
+ | |||
+ | Aim – Day 3 of Generating Competent Cells: Today do the final stages of the protocol, and should have a supply of competent cells. | ||
+ | |||
+ | (LOGO) – Day 3 of Generating Competent Cells | ||
+ | |||
+ | |||
+ | |||
<html></div> | <html></div> | ||
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Revision as of 15:27, 2 August 2012
Monday (18.6.12)
Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.
Method
(LOGO) – Day 1 of Generating Competent Cells
Tuesday (19.6.12)
Aim – Day 2 of Generating Competent Cells: Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation
(LOGO) – Day 2 of Generating Competent Cells
Wednesday (20.6.12)
Aim – Day 3 of Generating Competent Cells: Today do the final stages of the protocol, and should have a supply of competent cells.
(LOGO) – Day 3 of Generating Competent Cells