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Team:University College London/LabBook/Week3
From 2012.igem.org
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(LOGO) – Day 2 of Generating Competent Cells | (LOGO) – Day 2 of Generating Competent Cells | ||
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'''Aim – Permeabilisation:''' Incubating Cells in the presences of CaCl2 will prepare the cell wall, such that it becomes permeable to DNA. | '''Aim – Permeabilisation:''' Incubating Cells in the presences of CaCl2 will prepare the cell wall, such that it becomes permeable to DNA. | ||
Revision as of 15:32, 2 August 2012
Monday (18.6.12)
Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.
In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.
Method
(LOGO) – Day 1 of Generating Competent Cells
Tuesday (19.6.12)
Aim – Picking Colonies: Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation
(LOGO) – Day 2 of Generating Competent Cells
Wednesday (20.6.12)
Aim – Permeabilisation: Incubating Cells in the presences of CaCl2 will prepare the cell wall, such that it becomes permeable to DNA.
(LOGO) – Day 3 of Generating Competent Cells
