Team:University College London/LabBook/Week3

From 2012.igem.org

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(Monday (18.6.12))
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Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.
Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.
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Method
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In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.
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'''Method'''
(LOGO) – Day 1 of Generating Competent Cells
(LOGO) – Day 1 of Generating Competent Cells
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Tuesday (19.6.12)
 
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Aim – Day 2 of Generating Competent Cells: Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation
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== Tuesday (19.6.12) ==
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'''Aim – Picking Colonies:''' Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation
(LOGO) – Day 2 of Generating Competent Cells
(LOGO) – Day 2 of Generating Competent Cells
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Wednesday (20.6.12)
Wednesday (20.6.12)
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Aim – Day 3 of Generating Competent Cells: Today do the final stages of the protocol, and should have a supply of competent cells.  
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'''Aim – Permeabilisation:''' Incubating Cells in the presences of CaCl2 will prepare the cell wall, such that it becomes permeable to DNA.  
(LOGO) – Day 3 of Generating Competent Cells
(LOGO) – Day 3 of Generating Competent Cells

Revision as of 15:32, 2 August 2012

Monday (18.6.12)

Aim – Day 1 of Generating Competent Cells: In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.

In order to culture our BioBricks in a cell line, we must first enable our cells to uptake plasmid. Today we will be starting the first day of the protocol, whereby we streak the cells on a minimal agar.

Method

(LOGO) – Day 1 of Generating Competent Cells


Tuesday (19.6.12)

Aim – Picking Colonies: Today we check the colony formation from yesterday’s culture, and pick a colony for inoculation

(LOGO) – Day 2 of Generating Competent Cells

Wednesday (20.6.12)

Aim – Permeabilisation: Incubating Cells in the presences of CaCl2 will prepare the cell wall, such that it becomes permeable to DNA.

(LOGO) – Day 3 of Generating Competent Cells