Team:University College London/LabBook/Week14

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Contents

Monday 10.09.12

Aim: Day 2 of Generating competent cells. The aim is to incubate cells in the presence of CaCl2 to prepare the cell wall, such that it becomes permeable to DNA


Method: http://2012.igem.org/Team:University_College_London/Protocols/Competency2

Tuesday 11.09.12

Aim: Day 3 of Generating competent cells


Method: http://2012.igem.org/Team:University_College_London/Protocols/Competency3

14-2

14-3

14-4

Friday 14.09.13

Aim: To characterise nuclease activity


Method: To add protocol


Results:


The following table shows readings of the diameters and OD at different time points:


Date Time Colony diameter/mm Halo diameter/mm Absorbance at 600 OD
Friday 12.30 0 0 0
Friday 16.30 0 0 0
Friday 19.30 0 0 0
Friday 22.30 0 0 0
Saturday 01.30 0.5 1.5 0.068
Saturday 04.30 1 2 0.098
Saturday 07.30 1.5 3 0.159
Saturday 10.30 1.5 3.5 0.192
Saturday 12.30pm 2 4 0.203
Saturday 14.30 2 4 0.209
Saturday 16.30 2.5 5 0.215


The average depth of the colonies was noted to be 0.5 - 1mm


Conclusion: works bust in comparision to one found naturally in wnu it produces less nucles, as expected.