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Team:University College London/LabBook/Week14
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Contents |
Monday 10.09.12
Aim: Day 2 of Generating competent cells. The aim is to incubate cells in the presence of CaCl2 to prepare the cell wall, such that it becomes permeable to DNA
Method: https://2012.igem.org/Team:University_College_London/Protocols/Competency2
Tuesday 11.09.12
Aim: Day 3 of Generating competent cells
Method: https://2012.igem.org/Team:University_College_London/Protocols/Competency3
14-2
14-3
14-4
Friday 14.09.13
Aim: To characterise nuclease activity
Method:
To add protocol
Results:
The following table shows readings of the diameters and OD at different time points:
| Date | Time | Colony diameter/mm | Halo diameter/mm | Absorbance at 600 OD |
|---|---|---|---|---|
| Friday | 12.30 | 0 | 0 | 0 |
| Friday | 16.30 | 0 | 0 | 0 |
| Friday | 19.30 | 0 | 0 | 0 |
| Friday | 22.30 | 0 | 0 | 0 |
| Saturday | 01.30 | 0.5 | 1.5 | 0.068 |
| Saturday | 04.30 | 1 | 2 | 0.098 |
| Saturday | 07.30 | 1.5 | 3 | 0.159 |
| Saturday | 10.30 | 1.5 | 3.5 | 0.192 |
| Saturday | 12.30pm | 2 | 4 | 0.203 |
| Saturday | 14.30 | 2 | 4 | 0.209 |
| Saturday | 16.30 | 2.5 | 5 | 0.215 |
The average depth of the colonies was noted to be 0.5 - 1mm
Conclusion: works bust in comparision to one found naturally in wnu it produces less nucles, as expected.
