Team:University College London/LabBook/Week14

From 2012.igem.org

(Difference between revisions)
(Tuesday 11.09.12)
(14-5)
Line 35: Line 35:
</div><div class="experiment"></div><img id="14-5" src="http://2012.igem.org/wiki/images/2/29/Ucl2012-graph14-5.png" />
</div><div class="experiment"></div><img id="14-5" src="http://2012.igem.org/wiki/images/2/29/Ucl2012-graph14-5.png" />
<div class="experimentContent"></html>
<div class="experimentContent"></html>
-
==14-5==
+
==Friday 14.09.13==
 +
 
 +
 
 +
'''Aim:''' To characterise nuclease activity
 +
 
 +
 
 +
'''Method:'''
 +
To add protocol
 +
 
 +
 
 +
'''Results:'''
 +
 
 +
 
 +
The following table shows readings of the diameters and OD at different time points:
 +
 
 +
 
 +
{| class ="bigtable"
 +
|-
 +
! Date !! Time !! Colony diameter/mm !! Halo diameter/mm !! Absorbance at 600 OD
 +
|-
 +
|Friday || 12.30 || 0 || 0 || 0
 +
|-
 +
|Friday || 16.30 || 0 ||0 ||0
 +
|-
 +
|Friday || 19.30 || 0 ||0 ||0
 +
|-
 +
|Friday ||22.30 ||0 ||0 ||0
 +
|-
 +
|Saturday ||01.30 ||0.5 ||1.5 ||0.068
 +
|-
 +
|Saturday ||04.30 ||1 ||2 ||0.098
 +
|-
 +
|Saturday ||07.30 ||1.5 ||3 ||0.159
 +
|-
 +
|Saturday ||10.30 ||1.5 ||3.5 ||0.192
 +
|-
 +
|Saturday ||12.30pm ||2 ||4 ||0.203
 +
|-
 +
|Saturday ||14.30 ||2 ||4 ||0.209
 +
|-
 +
|Saturday || 16.30 ||2.5 ||5 ||0.215
 +
|}
 +
 +
 +
The average depth of the colonies was noted to be  0.5 - 1mm
 +
 
 +
 
 +
'''Conclusion:''' works bust in comparision to one found naturally in wnu it produces less nucles, as expected.
 +
 
 +
 
 +
 
<html></div>
<html></div>
<div class="experiment"></div></html>
<div class="experiment"></div></html>
{{:Team:University_College_London/templates/foot}}
{{:Team:University_College_London/templates/foot}}

Revision as of 00:27, 27 September 2012

Contents

Monday 10.09.12

Aim: Day 2 of Generating competent cells. The aim is to incubate cells in the presence of CaCl2 to prepare the cell wall, such that it becomes permeable to DNA


Method: http://2012.igem.org/Team:University_College_London/Protocols/Competency2

Tuesday 11.09.12

Aim: Day 3 of Generating competent cells


Method: http://2012.igem.org/Team:University_College_London/Protocols/Competency3

14-2

14-3

14-4

Friday 14.09.13

Aim: To characterise nuclease activity


Method: To add protocol


Results:


The following table shows readings of the diameters and OD at different time points:


Date Time Colony diameter/mm Halo diameter/mm Absorbance at 600 OD
Friday 12.30 0 0 0
Friday 16.30 0 0 0
Friday 19.30 0 0 0
Friday 22.30 0 0 0
Saturday 01.30 0.5 1.5 0.068
Saturday 04.30 1 2 0.098
Saturday 07.30 1.5 3 0.159
Saturday 10.30 1.5 3.5 0.192
Saturday 12.30pm 2 4 0.203
Saturday 14.30 2 4 0.209
Saturday 16.30 2.5 5 0.215


The average depth of the colonies was noted to be 0.5 - 1mm


Conclusion: works bust in comparision to one found naturally in wnu it produces less nucles, as expected.