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Team:UT Dallas/pop3 ag results
From 2012.igem.org
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We first checked whether each of the individual population of bacteria fluorosces. It seemed that both population 1 and population 2 worked fine. However, it seemed that there is slight leakage in population 3 as it is not supposed to fluorosce by itself.<br><br> | We first checked whether each of the individual population of bacteria fluorosces. It seemed that both population 1 and population 2 worked fine. However, it seemed that there is slight leakage in population 3 as it is not supposed to fluorosce by itself.<br><br> | ||
| - | <center><img src='https://static.igem.org/mediawiki/2012/ | + | <center><img src='https://static.igem.org/mediawiki/2012/0/00/And_gate_pic_1.png'></center> |
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Next, we tried combinations of different populations to make sure that population 3 works as it is supposed to. We tried the combination of population 1 and 3, population 2 and 3 and population 1,2, and 3.<br><br> | Next, we tried combinations of different populations to make sure that population 3 works as it is supposed to. We tried the combination of population 1 and 3, population 2 and 3 and population 1,2, and 3.<br><br> | ||
| - | <center><img src='https://static.igem.org/mediawiki/2012/ | + | <center><img src='https://static.igem.org/mediawiki/2012/e/e7/AND_gate_pic_3.png'></center> <br><br> |
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We tested these populations again by combining them in the same ratio. (100x)<br><br> | We tested these populations again by combining them in the same ratio. (100x)<br><br> | ||
| - | <center><img src='https://static.igem.org/mediawiki/2012/ | + | <center><img src='https://static.igem.org/mediawiki/2012/9/91/AND_gate_pic_2.png'></center> |
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We performed a final experiment to ensure the accuracy of our experiments by centrifuging both populations 1 and 2. Then we transferred the supernantant with quorum sensing molecules to the Population 3 test tube . While at the same time, we added the same amount of broth to the population 3 negative. And the following are the results: 40x<br><br> | We performed a final experiment to ensure the accuracy of our experiments by centrifuging both populations 1 and 2. Then we transferred the supernantant with quorum sensing molecules to the Population 3 test tube . While at the same time, we added the same amount of broth to the population 3 negative. And the following are the results: 40x<br><br> | ||
| - | <center><img src='https://static.igem.org/mediawiki/2012/ | + | <center><img src='https://static.igem.org/mediawiki/2012/f/f3/AND_gate_pic_4.png'></center> |
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Conclusion: From the above experiments, we can conclude that AND gate seems to work as expected except for some minor leakage of CFP possibly due to weak repression of plas.TetO by Tet R. | Conclusion: From the above experiments, we can conclude that AND gate seems to work as expected except for some minor leakage of CFP possibly due to weak repression of plas.TetO by Tet R. | ||
Revision as of 02:01, 4 October 2012
Three Population AND Gate Results
We first checked whether each of the individual population of bacteria fluorosces. It seemed that both population 1 and population 2 worked fine. However, it seemed that there is slight leakage in population 3 as it is not supposed to fluorosce by itself.
Next, we tried combinations of different populations to make sure that population 3 works as it is supposed to. We tried the combination of population 1 and 3, population 2 and 3 and population 1,2, and 3.
From this experiment, we came to the conclusion that our repressor TetR isn’t completely repressing plas.TetO in population 3, and hence we can see some CFP in the combination of population 2 & 3. However, CFP in population 1, 2 and 3 is brighter and defined , meaning that our system is working better when both population are present together.
We tested these populations again by combining them in the same ratio. (100x)
From the above experiment, we concluded that our AND Gate works except for some minor leakage when combined with cell population 2 (RFP).
We performed a final experiment to ensure the accuracy of our experiments by centrifuging both populations 1 and 2. Then we transferred the supernantant with quorum sensing molecules to the Population 3 test tube . While at the same time, we added the same amount of broth to the population 3 negative. And the following are the results: 40x
Conclusion: From the above experiments, we can conclude that AND gate seems to work as expected except for some minor leakage of CFP possibly due to weak repression of plas.TetO by Tet R.
