Team:UTK-Knoxville

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<b style="color:#4C4D4F;">Project Description </b>
<b style="color:#4C4D4F;">Project Description </b>
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How does an IRES work?  
How does an IRES work?  
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<p class="justify">However, many proteins are positioned as the second open reading frame in a strand of mRNA or contain highly structured 5’ untranslated regions, scanning from the 5’ end inefficient. This prevents the engineering of operons that contain two or more genes under the control of the same promoter. Luckily, nature has come up with its own remedy for this problem, known as IRES mediated translation initiation. This allows the ribosome to bind in the middle of a strand of mRNA and initiate translation. </p>
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What are we doing?  
What are we doing?  
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<p class="justify">However, many proteins are positioned as the second open reading frame in a strand of mRNA or contain highly structured 5’ untranslated regions, preventing efficient scanning from the 5’ end. This prevents the engineering of operons that contain two or more genes under the control of the same promoter. Luckily, nature has come up with its own remedy for this problem, known as IRES mediated translation initiation, which allows the ribosome to bind in the middle of a strand of mRNA and initiate translation. </p>
 
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Unfortunately, the Registry of Standard Biological Parts contains few IRESs and even those are poorly annotated. The UTK-Knoxville iGEM team hopes to begin to remedy this problem by characterizing a group of IRESs and submitting them to the registry. In addition, as a part of our characterization efforts, we plan to develop a methodology of determining relative IRES strength, as modeled by the methodology put forth by Kelly et al in the Journal of Biological Engineering (Kelly et al., 2001). Using flow cytometry, we will determine the relative levels of fluorescent protein under the control of each IRES, as expressed in S. cerevisiae. Through this work, we hope to initiate the growth of a library of IRESs and to develop a protocol to allow contribution by all members of the community.  
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Unfortunately, the Registry of Standard Biological Parts contains few IRESs and even those are poorly annotated. The UTK-Knoxville iGEM team hopes to remedy this problem by characterizing a group of IRESs and submitting them to the registry. In addition, we plan to develop a methodology of determining relative IRES strength, as modeled by the methodology put forth by Kelly et al in the Journal of Biological Engineering (Kelly et al., 2001). Using flow cytometry, we will determine the relative levels of fluorescent protein under the control of each IRES, as expressed in <i>S. cerevisiae</i>. Through this work we hope to initiate the growth of a library of IRESs and to develop a protocol to encourage contribution by all members of the community.</p>
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[https://igem.org/Main_Page iGEM Main Page]
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[[file:UTK_igem_group_photo.jpg|center|200px]]
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Visit our team page to get acquainted with our members!
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Want to get involved with iGEM at the University of Tennessee? We're recruiting members for he 2013 team! If you're interested, contact us at UTKiGEM@gmail.com.
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[[file:IRES_Power_T.jpg|left|315px]]

Latest revision as of 03:45, 4 October 2012

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