Team:UT-Tokyo/Safety

From 2012.igem.org

(Difference between revisions)
(safety Q&A 2)
 
(21 intermediate revisions not shown)
Line 1: Line 1:
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
{{:Team:UT-Tokyo/Template/Header|fullpagename=Team:UT-Tokyo/Safety|subpagename=Safety}}
{{:Team:UT-Tokyo/Template/Header|fullpagename=Team:UT-Tokyo/Safety|subpagename=Safety}}
 +
<html>
 +
</p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/4/41/UT_CB-20.jpg" alt="box-background image" /><p>
 +
<strong>Safety Q&amp;A:</strong> <br />
 +
Our answers to the questions posed at https://2012.igem.org/Safety are as follows
 +
</p></div><p>
 +
</html>
 +
= Safety Q&A =
Our answers to the questions posed at https://2012.igem.org/Safety are as follows:
Our answers to the questions posed at https://2012.igem.org/Safety are as follows:
;1. Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?
;1. Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?
-
:As long as we conduct experiments following the safety manual mentioned below (and of course we do follow) and obey the laws on biotechnology when conducting our experiments, we think our ideas raise no safety problems.
+
: As long as we conduct experiments following the safety manual of mentioned below (and of course we do follow) and obey the laws on biotechnology when conducting our experiments in a laboratory, we think our ideas raise no safety problems. <br /> Our safety manual is the the safety manual of the School of Engineering [http://safetymanual.t.u-tokyo.ac.jp/]. This is internal access only. So, if needed, please see the safety manual of the School of Science [http://www.s.u-tokyo.ac.jp/en/offices/esmo/manual.html], which is similar to that of the School of Engineering.
 +
:However, our final aim is to apply our project to actual garbage disposal. The ''E.coli'' we use must not be leaked to the environment because they are genetically modified organisms, which originally didn't exist in nature and so the impact on the eco-system by letting them in the wild is unknown and out of management. Besides, the synthesized H<sub>2</sub> gas needs to be separated from ''E.coli'' because, for example, it will be a problem if ''E.coli'' are mixed with a fuel cell using H<sub>2</sub>. This issue will be settled by using gas permeation membranes. Next, the way to deal with the waste that has used up its glucose, should be incineration. In case they are left for a long time, the possibility of undergoing mutation of ''E.coli'' becomes stronger and it is not clear what the mutated ''E.coli'' will do. (Even if we burn the waste at this step, the energy efficiency is higher than that to burn the waste before the the bacteria work on it, for ample energy is retreived.)
;2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
;2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
-
:No. The genes we use this year  are cloned from the E. coli JM109 strain and we think our bacteria are no more environmentally harmful nor evolutionarily advantageous than wild type ''E. coli''.
+
:The ''E.coli'' that were introduced the parts containing ''fhlA'' has more risk than Wild-Type ''E.coli'' because it will produce more H<sub>2</sub>, a flammable gas, than Wild-Type (depending on the environment).
 +
:However, the speed of H<sub>2</sub> synthesis was mild in our experiment and in prior research, so we think culturing them in LB for short periods of time does not raise a safety issue. The other parts are non-hazardous because they will lead only to fluorescent protein expression or will not be translated.
;3. Is there a local biosafety group, committee, or review board at your institution?
;3. Is there a local biosafety group, committee, or review board at your institution?
-
:Yes. The Noji laboratory at which we work follows the safety manual
+
: Yes. We work at Noji laboratory, School of Engineering, the University of Tokyo.<br />There are
-
[http://safetymanual.t.u-tokyo.ac.jp/] of the School of Engineering, University of Tokyo. This manual is internal access only, but it's similar to the safety manual of the School of Science[http://www.s.u-tokyo.ac.jp/en/offices/esmo/manual.html]. Our instructors from this lab think our project does not raise any safety problems.
+
:* Health and Safety Office
 +
:** Committee on Genetically Modified Organisms and Microbes for Research
 +
:** Research Ethics Committee
 +
: in the School of Engineering, and there is
 +
:* Office for Life Science Research Ethics and Safety [http://www.u-tokyo.ac.jp/ja/administration/lifescience/]
 +
: in the University of Tokyo.
;4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
;4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
:If there are more strictly defined rules and evaluations for safety, we believe they will raise the safety level of this competition and be useful in an educational and academic sense.
:If there are more strictly defined rules and evaluations for safety, we believe they will raise the safety level of this competition and be useful in an educational and academic sense.
 +
<!-- 以上自由記述 -->
<html>
<html>
-
<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
+
</div></div><p>
-
<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
+
-
This is a template page. READ THESE INSTRUCTIONS.
+
-
</div>
+
-
<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
+
-
</div>
+
-
<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
+
-
You <strong>MUST</strong>  have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace. 
+
-
</div>
+
-
</div>
+
</html>
</html>
 +
{{:Team:UT-Tokyo/Template/Side|twdisp=block}}
 +
<!-- 以下サイドメニュー編集 ;の後には、もしこのページが第2階層にあれば、そのページ内の見出しを羅列。第1階層にあれば、そのカテゴリ内の第2階層ページ名を羅列する。;の後には名称を、:の後にはその説明を記述する。:と;のセットは適当に増減してください -->
 +
;[[#Safety_Q.26A|Safety Q&A]]
 +
:to answer the questions on the [https://2012.igem.org/Safety safety page.]
-
<!-- *** End of the alert box *** -->
+
<!-- ここまでサイドメニュー編集を -->
-
 
+
<html></p>
-
 
+
</div>
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
</div><p>
-
!align="center"|[[Team:UT-Tokyo|Home]]
+
</html>
-
!align="center"|[[Team:UT-Tokyo/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2012&team_name=UT-Tokyo Official Team Profile]
+
-
!align="center"|[[Team:UT-Tokyo/Project|Project]]
+
-
!align="center"|[[Team:UT-Tokyo/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:UT-Tokyo/Modeling|Modeling]]
+
-
!align="center"|[[Team:UT-Tokyo/Notebook|Notebook]]
+
-
!align="center"|[[Team:UT-Tokyo/Safety|Safety]]
+
-
!align="center"|[[Team:UT-Tokyo/Attributions|Attributions]]
+
-
|}
+
-
 
+
-
 
+
-
Use this page to answer the questions on the  [[Safety | safety page]].
+
-
{{:Team:UT-Tokyo/Template/Footer|fullpagename=Team:UT-Tokyo/Safety|subpagename=Safety|nextname=|prevname=}}
+
{{:Team:UT-Tokyo/Template/Footer|prevfull=Notebook|nextfull=HumanPractice|nextname= HumanPractice|prevname=Notebook}}

Latest revision as of 16:43, 3 October 2012

Safety

box-background image

Safety Q&A:
Our answers to the questions posed at https://2012.igem.org/Safety are as follows

Safety Q&A

Our answers to the questions posed at https://2012.igem.org/Safety are as follows:

1. Would any of your project ideas raise safety issues in terms of researcher safety, public safety, or environmental safety?
As long as we conduct experiments following the safety manual of mentioned below (and of course we do follow) and obey the laws on biotechnology when conducting our experiments in a laboratory, we think our ideas raise no safety problems.
Our safety manual is the the safety manual of the School of Engineering [1]. This is internal access only. So, if needed, please see the safety manual of the School of Science [2], which is similar to that of the School of Engineering.
However, our final aim is to apply our project to actual garbage disposal. The E.coli we use must not be leaked to the environment because they are genetically modified organisms, which originally didn't exist in nature and so the impact on the eco-system by letting them in the wild is unknown and out of management. Besides, the synthesized H2 gas needs to be separated from E.coli because, for example, it will be a problem if E.coli are mixed with a fuel cell using H2. This issue will be settled by using gas permeation membranes. Next, the way to deal with the waste that has used up its glucose, should be incineration. In case they are left for a long time, the possibility of undergoing mutation of E.coli becomes stronger and it is not clear what the mutated E.coli will do. (Even if we burn the waste at this step, the energy efficiency is higher than that to burn the waste before the the bacteria work on it, for ample energy is retreived.)
2. Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?
The E.coli that were introduced the parts containing fhlA has more risk than Wild-Type E.coli because it will produce more H2, a flammable gas, than Wild-Type (depending on the environment).
However, the speed of H2 synthesis was mild in our experiment and in prior research, so we think culturing them in LB for short periods of time does not raise a safety issue. The other parts are non-hazardous because they will lead only to fluorescent protein expression or will not be translated.
3. Is there a local biosafety group, committee, or review board at your institution?
Yes. We work at Noji laboratory, School of Engineering, the University of Tokyo.
There are
  • Health and Safety Office
    • Committee on Genetically Modified Organisms and Microbes for Research
    • Research Ethics Committee
in the School of Engineering, and there is
  • Office for Life Science Research Ethics and Safety [3]
in the University of Tokyo.
4. Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
If there are more strictly defined rules and evaluations for safety, we believe they will raise the safety level of this competition and be useful in an educational and academic sense.

Safety Q&A
to answer the questions on the safety page.