Team:UT-Tokyo/Project/Inhibition

From 2012.igem.org

(Difference between revisions)
Line 9: Line 9:
<!-- 以下テンプレ部分まで自由記述 -->
<!-- 以下テンプレ部分まで自由記述 -->
-
== 編集の仕方 ==
+
== Abstract ==
-
* ページ右上にあるログインリンクからログインできます。
+
In project H<sub>2</sub> ''E.coli'', we overexpressed fhlA in an attempt to increase the amount of hydrogen production. However, ''E.coli'' possess a protein called HycA in their genome preventing unrestricted hydrogen synthesis. So, here we intended to inhibit the activity of HycA in order to enhance H2 production. In doing so, we did not want to knock out the gene from the ''E. coli'' genome as this will restrict the bacterial strain the completed part can be used in.
-
* ログイン済みの場合は、ページ左上にカーソルを持っていけば、editから内容を編集できます。(日本語メニューの場合は「編集」)
+
-
* 新規ページを作るには、アドレスバーに作りたいページのURLを打ち込めばできます。そのページには、このテンプレートページの内容を全てコピーして貼り付け、指定がある部分を編集して自由記述すればOKです。
+
-
== wikiの記法 ==
+
We adopted a strategy to sequester cellular HycA by overproduction of molecules that bind to this protein, therefore preventing it from performing its repressive function.
-
[http://2008.igem.org/Team:Chiba/Internal/foredit 2008年度Team:Chibaのwiki]が参考になります。
+
For example, if HycA works as a transcriptional repressor, then the nucleic acid sequence which HycA binds to is amplified and introduced into a high-copy plasmid as a multiple-tandem repeat.
-
そのページでもリンクされていますが、書き方は
+
-
[http://ja.wikipedia.org/wiki/Help:%E3%83%9A%E3%83%BC%E3%82%B8%E3%81%AE%E7%B7%A8%E9%9B%86 Wikipedia-Help:ページの編集]準拠のようです。
+
-
* 表の書き方:[http://ja.wikipedia.org/wiki/Help:%E8%A1%A8%E3%81%AE%E4%BD%9C%E3%82%8A%E6%96%B9 wikipedia Help:表の作り方]
+
However, the mechanism with which HycA prevents hydrogen synthesis, as well as any DNA sequences it may bind to is unclear and we will need to determine this in order for this strategy to work. In the meantime, we set out to explore whether the strategy in general is usable to knock-down gene expression.
-
== 見出し1 ==
+
To this end we used LacI, AraC and ArgR whose binding sites are known, and introduced multiple copies of their binding sites into plasmids to examine how the expression levels of genes downstream of these proteins were affected.
-
== 見出し2 ==
+
The results was ...
-
=== 小見出し1 ===
+
-
リンクの例:<br />
+
This method is applicable to transcriptional factors in general. It is possible that we can control only the strength of the matching promoter, without changing the sequence of the promoter, by varying the number of the repeats of the binding sites to be introduced.
-
*ページ内リンク
+
-
**右のように[[#見出し1]]と書くと見出し1へのリンクが張られます。ページトップ[[#top]]と、各見出しへはこのようにしてリンクが張れます。
+
-
*Wiki内部リンク
+
-
**右のように[[Team:UT-Tokyo/Internal/Sandbox]] と書くとそのまま表示され<br />
+
-
**右のように[[Team:UT-Tokyo/Internal/Sandbox|Sandboxへのリンク]]と書くと「Sandboxへのリンク」という文字列にリンクが張られます。
+
-
*Wiki外部リンク
+
-
**右のようにhttp://2012.igem.org/Team:UT-Tokyoと書くとそのまま表示され、
+
-
**右のように[http://2012.igem.org/Team:UT-Tokyo UT-Tokyoのトップページ]と書くと「UT-Tokyoのトップページ」という文字列にリンクが張られます。
+
-
**右のように[http://2012.igem.org/Team:UT-Tokyo]と書くと、自動で番号のついたリンクが張られます。
+
-
画像にリンクしたい場合:
+
Moreover, this method allows for the regulation of expression of genes present in the bacterial genome, something that has been challenging when dealing with BioBricks due to standardization issues.
-
[[media:example.jpg]]
+
-
 
+
-
画像を表示したい場合
+
-
[[File:ファイル名(拡張子込)]]
+
-
 
+
-
 
+
-
段落内改行は<br />
+
-
 
+
-
;定義リストの定義
+
-
:定義リストの説明
+
-
 
+
-
== 画像類 ==
+
-
 
+
-
[[Team:UT-Tokyo/Internal/Images]]にがぞうのせてる
+
<!-- 以上自由記述 -->
<!-- 以上自由記述 -->

Revision as of 06:11, 26 September 2012

Inhibition without Knockout

box-background image

このページの概要を、簡単に記述。

Abstract

In project H2 E.coli, we overexpressed fhlA in an attempt to increase the amount of hydrogen production. However, E.coli possess a protein called HycA in their genome preventing unrestricted hydrogen synthesis. So, here we intended to inhibit the activity of HycA in order to enhance H2 production. In doing so, we did not want to knock out the gene from the E. coli genome as this will restrict the bacterial strain the completed part can be used in.

We adopted a strategy to sequester cellular HycA by overproduction of molecules that bind to this protein, therefore preventing it from performing its repressive function.

For example, if HycA works as a transcriptional repressor, then the nucleic acid sequence which HycA binds to is amplified and introduced into a high-copy plasmid as a multiple-tandem repeat.

However, the mechanism with which HycA prevents hydrogen synthesis, as well as any DNA sequences it may bind to is unclear and we will need to determine this in order for this strategy to work. In the meantime, we set out to explore whether the strategy in general is usable to knock-down gene expression.

To this end we used LacI, AraC and ArgR whose binding sites are known, and introduced multiple copies of their binding sites into plasmids to examine how the expression levels of genes downstream of these proteins were affected.

The results was ...

This method is applicable to transcriptional factors in general. It is possible that we can control only the strength of the matching promoter, without changing the sequence of the promoter, by varying the number of the repeats of the binding sites to be introduced.

Moreover, this method allows for the regulation of expression of genes present in the bacterial genome, something that has been challenging when dealing with BioBricks due to standardization issues.

見出し1or子下階層ページ名1
題名1の説明
改行して説明の続き
見出し2or子階層ページ名2
説明
見出し2or子階層ページ名3
description
見出し2or子階層ページ名4
description