Team:UT-Tokyo/Project/H2 E.coli/Results

From 2012.igem.org

(Difference between revisions)
Line 4: Line 4:
</p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/7/78/UT_Tokyo_0917_0249.jpg" alt="box-background image" /><p>
</p><img id="abstimg" src="https://static.igem.org/mediawiki/2012/7/78/UT_Tokyo_0917_0249.jpg" alt="box-background image" /><p>
<!-- ここから</p>の前までを編集してください -->
<!-- ここから</p>の前までを編集してください -->
-
このページの概要を、簡単に記述。
+
Here our experimental results are given.
</p></div><p></html>
</p></div><p></html>
<!-- 以下テンプレ部分まで自由記述 -->
<!-- 以下テンプレ部分まで自由記述 -->
== Result ==
== Result ==
-
We measured the amount of H<sub>2</sub> produced by ''E.coli'' transformed with either “pLac-RBS-fhlA-d.term”(''fhlA'') or “pLac-RBS-fhlA(E363G)-d.term”(E363G)  by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. In this page, we describe the data.
+
We measured the amount of H<sub>2</sub> produced by ''E.coli'' transformed with either “pLac-RBS-fhlA-d.term”(''fhlA'') or “pLac-RBS-fhlA(E363G)-d.term”(''fhlA'' (E363G))  by the method written in the page [[Team:UT-Tokyo/LabWork/AssayMethods|AssayMethods]]. <br />
 +
In this page, we describe the data.
The result is  the following (Fig.1)<br />
The result is  the following (Fig.1)<br />
Line 18: Line 19:
The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br />
The results shown by the Fig.1 is statistically sufficient to conclude the followings.<br />
-
*The amount of H<sub>2</sub> production is ''fhlA'' > ''fhlA''(E363G) > wild type in these conditions.
+
*The amount of H<sub>2</sub> production is ''fhlA'' > ''fhlA'' (E363G) > wild type in these conditions.
*There is a positive correlation between the amount of H<sub>2</sub> produced by ''fhlA'' and the glucose concentration in the liquid LB.
*There is a positive correlation between the amount of H<sub>2</sub> produced by ''fhlA'' and the glucose concentration in the liquid LB.
   
   

Revision as of 23:02, 26 September 2012

H2 E.coli: Results

box-background image

Here our experimental results are given.

Result

We measured the amount of H2 produced by E.coli transformed with either “pLac-RBS-fhlA-d.term”(fhlA) or “pLac-RBS-fhlA(E363G)-d.term”(fhlA (E363G)) by the method written in the page AssayMethods.
In this page, we describe the data.

The result is the following (Fig.1)

UT Tokyo Fig diagram 640 480.png

Fig.1:H2production in liquid LB medium containing the labeled glucose concentration.
The vertical axis expresses v/v% of H2 in the gaseous phase after we cultivate each E.coli for 8 hours in a container with 1mL each of LB medium and the gaseous phase .The horizontal axis shows the kinds of E.coli and the concentrations of glucose in each medium(“LB+x%” means the glucose w/v concentrations of liquid LB medium is x% more than the usual medium.

The results shown by the Fig.1 is statistically sufficient to conclude the followings.

  • The amount of H2 production is fhlA > fhlA (E363G) > wild type in these conditions.
  • There is a positive correlation between the amount of H2 produced by fhlA and the glucose concentration in the liquid LB.

The detailed interpretations of this result are done in the Discussion.