Team:UT-Tokyo/Notebook

From 2012.igem.org

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<p></html>
==== May 29th ====
==== May 29th ====
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*making LB medium
+
*Making LB medium
==== May 30th ====
==== May 30th ====
-
*making LB Agar
+
*Making LB Agar
-
*making LB Amp Plates
+
*Making LB Amp Plates
==== May 31st ====
==== May 31st ====
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*preculture
+
*Preculture
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==== June 5th ====
==== June 5th ====
-
*plating JM109
+
*Plating JM109
==== June 6th ====
==== June 6th ====
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==== June 7th ====
==== June 7th ====
-
*measuring OD by spectroscope
+
*Measuring OD by spectroscope
-
*meking electrocompetent cells
+
*Meking electrocompetent cells
-
*electroporating of a test plasmid
+
*Electroporating of a test plasmid
==== June 8th ====
==== June 8th ====
-
*caluculatiing transformation efficiency
+
*Caluculatiing transformation efficiency
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<div id="Week03" class="Weeks">
<div id="Week03" class="Weeks">
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==== m dd ====
 
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==== m dd ====
 
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==== June 26th ====
==== June 26th ====
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*making LB tetracycline plate
+
*Making LB tetracycline plate
==== June 27th ====
==== June 27th ====
==== June 28th ====
==== June 28th ====
-
*making LB kanamycin plate and chloramphenicol plate
+
*Making LB kanamycin plate and chloramphenicol plate
-
*transformation with a test plasmid
+
*Transformation with a test plasmid
==== June 29th ====
==== June 29th ====
-
*transformation with #1(constitutive promoter) and #11(RBS)
+
*Transformation with #1(constitutive promoter) and #11(RBS)
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<h3 onclick="ShowNote('Week06');" onkeypress="ShowNote('Week06');">6th week: 7/1-7/7</h3>
<h3 onclick="ShowNote('Week06');" onkeypress="ShowNote('Week06');">6th week: 7/1-7/7</h3>
<div id="Week06" class="Weeks">
<div id="Week06" class="Weeks">
 +
====  July 3rd====
 +
*Making K solution
 +
*Colony PCR DNA fragment assumed as fhlA (#6) for cloning
 +
 +
==== July 4th ====
 +
*Miniprep #1, #11
 +
 +
==== July 5th ====
 +
*Making 1% agarose solution
 +
*Electrophoresis (#6)
 +
*Digest (#1_EP, #11-_EP)
 +
 +
==== July 6th ====
 +
*Colony PCR (#6)
 +
</div>
 +
 +
 +
 +
<h3 onclick="ShowNote('Week07');" onkeypress="ShowNote('Week07');">7th week: 7/8-7/14</h3>
 +
<div id="Week07" class="Weeks">
 +
==== July 9th ====
 +
*Electrophoresis (#6)
 +
 +
==== July 10th ====
 +
*Transformation
 +
**#3(RBS medium), #5(d.term), #20(pLac)
 +
 +
==== July 11th ====
 +
*Preculture
 +
** #3, #5, #20
 +
*Gel Extraction(#6)
 +
 +
==== July 12th ====
 +
*The continuation of Gel Extraction(#6)
 +
*Miniprep
 +
** #3, #5, #20
 +
 +
</div>
 +
 +
 +
 +
<h3 onclick="ShowNote('Week08');" onkeypress="ShowNote('Week08');">8th week: 7/15-7/21</h3>
 +
<div id="Week08" class="Weeks">
 +
</div>
 +
 +
 +
 +
<h3 onclick="ShowNote('Week09');" onkeypress="ShowNote('Week09');">9th week: 7/22-7/28</h3>
 +
<div id="Week09" class="Weeks">
</div>
</div>

Revision as of 04:25, 26 September 2012

Notebook

box-background image

このページの概要を、簡単に記述。


May

1st week: 5/27-6/2

May 29th

  • Making LB medium

May 30th

  • Making LB Agar
  • Making LB Amp Plates


May 31st

  • Preculture


June

2nd week: 6/3-6/9

June 5th

  • Plating JM109

June 6th

  • preculture

June 7th

  • Measuring OD by spectroscope
  • Meking electrocompetent cells
  • Electroporating of a test plasmid

June 8th

  • Caluculatiing transformation efficiency


3rd week: 6/10-6/16

4th week: 6/17-6/23

5th week: 6/24-6/30

==== June 26th ==== *Making LB tetracycline plate ==== June 27th ==== ==== June 28th ==== *Making LB kanamycin plate and chloramphenicol plate *Transformation with a test plasmid ==== June 29th ==== *Transformation with #1(constitutive promoter) and #11(RBS)

2012/07

6th week: 7/1-7/7

==== July 3rd==== *Making K solution *Colony PCR DNA fragment assumed as fhlA (#6) for cloning ==== July 4th ==== *Miniprep #1, #11 ==== July 5th ==== *Making 1% agarose solution *Electrophoresis (#6) *Digest (#1_EP, #11-_EP) ==== July 6th ==== *Colony PCR (#6)

7th week: 7/8-7/14

==== July 9th ==== *Electrophoresis (#6) ==== July 10th ==== *Transformation **#3(RBS medium), #5(d.term), #20(pLac) ==== July 11th ==== *Preculture ** #3, #5, #20 *Gel Extraction(#6) ==== July 12th ==== *The continuation of Gel Extraction(#6) *Miniprep ** #3, #5, #20

8th week: 7/15-7/21

9th week: 7/22-7/28

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