http://2012.igem.org/wiki/index.php?title=Team:USC&feed=atom&action=historyTeam:USC - Revision history2024-03-28T11:11:20ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:USC&diff=268036&oldid=prevSeancurran at 03:57, 4 October 20122012-10-04T03:57:46Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> </div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><br></ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p><strong>Special thanks to:</strong></p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p> University of Southern California Undergraduate Research Programs</p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p> Davis School of Gerontology </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p> Viterbi School of Engineering </p></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><p> Dornsife College of Letters, Arts, and Sciences</p></ins></div></td></tr>
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</table>Seancurranhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=266332&oldid=prevSeancurran at 03:08, 4 October 20122012-10-04T03:08:26Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify how environmental conditions effect the bacterium <em>E. coli</em> by engineering a system that produces a predictable response in a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. <del class="diffchange diffchange-inline">Control </del>of these genes can be regulated by creating various combinations of genetic promoters which <del class="diffchange diffchange-inline">control expression and </del>activity of individual components of the flagella <del class="diffchange diffchange-inline">mechanism</del>. By promoting the <em>E. coli</em> flagella genes <del class="diffchange diffchange-inline">in various </del>conditions, such as salt concentration, nitrate concentration, pH and temperature, we can <del class="diffchange diffchange-inline">prove </del>a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental <del class="diffchange diffchange-inline">conditions </del>(see below). Thus, by specifying the bacteria’s response to an <del class="diffchange diffchange-inline">environment </del>on a genetic level, the resulting frequency can be used as a convenient assay <del class="diffchange diffchange-inline">for future researchers </del>to determine the <del class="diffchange diffchange-inline">response </del>of <del class="diffchange diffchange-inline">their test subjects to a controlled environment</del>.</span></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify how environmental conditions effect the bacterium <em>E. coli</em> by engineering a system that produces a predictable response in a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. <ins class="diffchange diffchange-inline"> Expression </ins>of these genes can be regulated by creating various combinations of genetic promoters<ins class="diffchange diffchange-inline">, </ins>which <ins class="diffchange diffchange-inline">modulate the </ins>activity of individual components of the flagella <ins class="diffchange diffchange-inline">apparatus</ins>. By promoting the <em>E. coli</em> flagella genes <ins class="diffchange diffchange-inline">under varying </ins>conditions, such as salt concentration, nitrate concentration, pH and temperature, we can <ins class="diffchange diffchange-inline">identify </ins>a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on <ins class="diffchange diffchange-inline">the </ins>varied environmental <ins class="diffchange diffchange-inline">condition </ins>(see below). Thus, by specifying the bacteria’s response to an <ins class="diffchange diffchange-inline">environmental change </ins>on a genetic level, the resulting frequency can be used as a convenient assay to determine the <ins class="diffchange diffchange-inline">real time status </ins>of <ins class="diffchange diffchange-inline">the culture</ins>.</span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td></tr>
</table>Seancurranhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=266212&oldid=prevSeancurran at 03:04, 4 October 20122012-10-04T03:04:32Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify how environmental conditions effect the bacterium <em>E. coli</em>by engineering a system that produces a predictable response in a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify how environmental conditions effect the bacterium <em>E. coli</em> by engineering a system that produces a predictable response in a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td></tr>
</table>Seancurranhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=266201&oldid=prevSeancurran at 03:04, 4 October 20122012-10-04T03:04:16Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify <del class="diffchange diffchange-inline">the </del>environmental conditions <del class="diffchange diffchange-inline">and their </del>effect <del class="diffchange diffchange-inline">on </del>the <del class="diffchange diffchange-inline">bacteria </del><em>E. coli</em> by engineering a system that <del class="diffchange diffchange-inline">causes </del>a predictable response <del class="diffchange diffchange-inline">to </del>a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify <ins class="diffchange diffchange-inline">how </ins>environmental conditions effect the <ins class="diffchange diffchange-inline">bacterium </ins><em>E. coli</em>by engineering a system that <ins class="diffchange diffchange-inline">produces </ins>a predictable response <ins class="diffchange diffchange-inline">in </ins>a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td></tr>
</table>Seancurranhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=265306&oldid=prevRkohan at 02:30, 4 October 20122012-10-04T02:30:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> E.&nbsp;Musici </h2><!-- .postitle --></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> E.&nbsp;Musici </h2><!-- .postitle --></div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"><href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3</del>.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
</table>Rkohanhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=265292&oldid=prevRkohan at 02:30, 4 October 20122012-10-04T02:30:21Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <<del class="diffchange diffchange-inline">p </del>style="text-align:left;"><<del class="diffchange diffchange-inline">a </del>href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <style="text-align:left;"><href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><<del class="diffchange diffchange-inline">a </del>href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
</table>Rkohanhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=265216&oldid=prevRkohan at 02:27, 4 October 20122012-10-04T02:27:29Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
</table>Rkohanhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=265199&oldid=prevRkohan at 02:26, 4 October 20122012-10-04T02:26:50Z<p></p>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div> <p style="text-align:left;"><a href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div> <p style="text-align:left;"><a href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138<ins class="diffchange diffchange-inline">.gif?w=960" alt="" /></a></ins></div></td></tr>
<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline"><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3</ins>.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
</table>Rkohanhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=263808&oldid=prevEllenPark at 01:26, 4 October 20122012-10-04T01:26:51Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E.coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E.coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E.coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E.coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E. coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E. coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E. coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E. coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions (see below). Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td></tr>
</table>EllenParkhttp://2012.igem.org/wiki/index.php?title=Team:USC&diff=263486&oldid=prevSeancurran at 01:11, 4 October 20122012-10-04T01:11:20Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p style="text-align:left;"><a href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> <p style="text-align:left;"><a href="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif"><img class="aligncenter size-full wp-image-180" title="tumble" src="http://uscigem2012.files.wordpress.com/2012/07/2172138.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:center;"><span style="color:#000000;"><strong>Abstract</strong></span></p></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E.coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E.coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E.coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E.coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions. Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><p style="text-align:justify;"><span style="color:#000000;">Our project aims to create a new system to quantify the environmental conditions and their effect on the bacteria <em>E.coli</em> by engineering a system that causes a predictable response to a controlled environment. Many strains of <em>E.coli</em> possess flagella, a whip-like appendage that can be found protruding from both eukaryotic and prokaryotic single-celled organisms. The assembly and motor control of <em>E.coli</em> flagella is under the control of a key group of genetic factors primarily for flagella assembly and chemotactic control. Control of these genes can be regulated by creating various combinations of genetic promoters which control expression and activity of individual components of the flagella mechanism. By promoting the <em>E.coli</em> flagella genes in various conditions, such as salt concentration, nitrate concentration, pH and temperature, we can prove a significant change in flagella rotation and frequency. Ultimately, this frequency can be translated into an audible range which can act as an indicator of the bacteria’s distress based on varied environmental conditions <ins class="diffchange diffchange-inline">(see below)</ins>. Thus, by specifying the bacteria’s response to an environment on a genetic level, the resulting frequency can be used as a convenient assay for future researchers to determine the response of their test subjects to a controlled environment.</span></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p><a href="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif"><img class="aligncenter size-full wp-image-259" title="Singing_Bacteria" src="http://uscigem2012.files.wordpress.com/2012/07/singing_bacteria3.gif?w=960" alt="" /></a></p></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div><p style="text-align:justify;"><a href="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg"><img class="aligncenter size-full wp-image-234" title="USC_logo" src="http://uscigem2012.files.wordpress.com/2012/07/usc_logo.jpg?w=960" alt="" /></a></p></div></td></tr>
</table>Seancurran