Team:UNAM Genomics Mexico/prueba

From 2012.igem.org

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{{:Template:Team:UNAM_Genomics_Mexico/webhtml| content=
{{:Template:Team:UNAM_Genomics_Mexico/webhtml| content=
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= MAY =
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<div class='thumbnailWrapper'>
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<ul>
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<img src='https://static.igem.org/mediawiki/2012/5/5c/UnamgenomicsoANDcadmio09_14_2012.jpg' width="300"/>
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<div class='captiongray'>
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<p class='captionInside'>1.ArsR-CzrA 97 E/P. <br />
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2.ArsR-CzrA 98 E/P. <br />
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3.ArsR-CzrA 99 E/P. <br />
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4.CI 02 E/P. <br />
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5.P4 PCR. <br />
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</div>
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</li>
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</ul>
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<br /><br />
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<img src='https://static.igem.org/mediawiki/2012/0/04/UnamgenomicsoANDcadmio09_14_2012_2.jpg' height="300"/>
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<div class='captionnaranja'>
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<p class='captionInside'>We extracted the correct band by kit <br />
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1.ArsR-CzrA 97 E/P. <br />
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2.ArsR-CzrA 98 E/P. <br />
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3.ArsR-CzrA 99 E/P. <br />
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4.CI 02 E/P. <br />
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5.P4 PCR. <br />
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</div>
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</li>
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</ul>
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</div>
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<br /><br />
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</html>
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<div class='thumbnailWrapper'>
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<img src='https://static.igem.org/mediawiki/2012/e/ea/UnamgenomicsoANDcadmio09_14_2012_3.jpg' height="300"/>
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<div class='captionrojo'>
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<p class='captionInside'>1. 1 kb ladder. <br />
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2.00179 Lasr lysis<br />.
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3.00179 LasR. <br />
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4.00179 Lasr (-).<br />
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5.LasR 00179 new primers. <br />
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6.LasR 00179 new primers. <br />
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7.LasR 00179 new primers (-).<br />
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</div>
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</li>
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</ul>
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<br /><br />
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<div class='thumbnailWrapper'>
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<li>
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<img src='https://static.igem.org/mediawiki/2012/9/9e/UnamgenomicsoANDcadmio09_14_2012_4.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>1.1 kb ladder. <br />
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3.ArsR-CzrA 97 E/P. <br />
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5.ArsR-CzrA 98 E/P. <br />
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7.ArsR-CzrA 99 E/P. <br />
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9.CI 02 E/P. <br />
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11.AmyE 5’ ArsR-CzrA 97 E/S. <br />
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13.AmyE 5’ ArsR-CzrA 98 E/S. <br />
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15.AmyE 5’ ArsR-CzrA 99 E/S. <br />
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</div>
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</ul>
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<img src='https://static.igem.org/mediawiki/2012/5/55/UnamgenomicsoANDcadmio09_14_2012_5.jpg' height="300"/>
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<div class='captionrosa'>
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<p class='captionInside'>1.ArsR-CzrA 97 E/P. <br />
 +
2.ArsR-CzRA 98 E/P. <br />
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3.1 kb ladder. <br />
 +
4.ArsR-CzRA 99 E/P. <br />
 +
5.CI 02 E/P. <br />
 +
6.AmyE 5’ 97 E/S. <br />
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7.AmyE 5’ 98 E/S. <br />
 +
8.1 kb ladder. <br />
 +
9.AmyE 5’ 99 E/S. <br />
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10.LasR PCR. <br />
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</div>
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</li>
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</ul>
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</div>
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<br /><br />
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</html>
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<div class='thumbnailWrapper'>
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<ul>
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<img src='https://static.igem.org/mediawiki/2012/2/2f/UnamgenomicsoANDcadmio09_14_2012_6.jpg' height="300"/>
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<div class='captionaqua'>
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<p class='captionInside'>1.ArsR-CzrA 97 E/P. <br />
 +
2.ArsR-CzRA 98 E/P. <br />
 +
3.1 kb ladder. <br />
 +
4.ArsR-CzRA 99 E/P. <br />
 +
5.CI 02 E/P. <br />
 +
6.AmyE 5’ 97 E/S. <br />
 +
7.AmyE 5’ 98 E/S. <br />
 +
8.1 kb ladder. <br />
 +
9.AmyE 5’ 99 E/S. <br />
 +
10.LasR PCR. <br />
 +
</div>
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</li>
 +
</ul>
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</div>
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<br /><br />
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</html>
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<h2>09/14/12</h2><br />
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We extracted the correct band by kit [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Gel_extraction_protocol | GEL EXTRACTION PROTOCOL]]. <br /><br />
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>We transformed ligation psB1c3+ArsR-CzrA 97/98/99/CI in competent DH5α cells from the pBad/pXyl AND team and in our own DH5α competent cells[[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Transformation | TRANSFORMATION PROTOCOL]]. <br /><br />
<br />
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<h1>'''Nanotubes'''</h1>
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<td id="leftcolumn2" align="center"><img src="https://static.igem.org/mediawiki/2012/d/d2/Unamgenomicsnanotubes.jpg" alt="some_text" height="200"/><br /><br /><p>Nanotubes</p></td>
 
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<td  id="contentcolumn2" align="center"><img src="https://static.igem.org/mediawiki/2012/9/96/Unamgenomicsnanotubes1.jpg" alt="some_text" height="200"/><br /><br /><p>Nanotubes</p></td>
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<td id="rightcolumn2"><img src="https://static.igem.org/mediawiki/2012/c/cd/UnamgenomicsLogiv.jpg" alt="some_text" height="190"/><br /><br /><p>Nanotubes</p></td>
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<br />
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Just as people, bacterium need to communicate with others, for this purpose there are many ways, and one of the more recent discovery are Nanotubes that bridge neighboring cells, providing a network for exchange of cellular molecules within and between species. Ben-Yehuda et al discovered these nanotubes in 2011, they show and extraordinary form of communication between Bacillus subtilis. Our team was astonished because of the implications, in the article is described that GFP and calcein, two molecules which cannot leave the cytoplasm, can be transferred to neighboring cells in B. subtillis, it means bacterium can share cytoplasm, the complex network of cells sharing cytoplasm that can be created was our main motivation to create Bacillus Booleanus.<br />
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<br />
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ItÕs still little known about nanotubes, and thatÕs make them a very interesting subject of study, and we are not the first iGEM team interested in working with them, in 2011 Paris_Bettencourt team work with the nanotubes, their goal was to characterize them.<br />
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To accomplish our goal of communicate our logic gates, we contacted Ben Yehuda group, and Paris Bettencourt iGEM 2011 team, to obtain protocols and work experience.<br />
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<br />
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We work to recreate the formation of nanotubes, but it was impossible to see them because the scanning microscope we have access to, has not enough resolution to see them.<br />
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<h2>09/15/12</h2><br />
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>We checked our transformations an did liquid cultures and passed the colonies to new plates [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Liquid_culture | LIQUID CULTURE PROTOCOL]]. <br />
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>We digested E0040+B0014 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Digestion_protocol_.2820_.C2.B5l.29 | DIGESTIONS PROTOCOL]]. <br />
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>We ligated 97/98/99 psBIC3 [[Team:UNAM_Genomics_Mexico/Notebook/Protocols#Ligation | LIGATION PROTOCOL]]. <br />
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<img src='https://static.igem.org/mediawiki/2012/6/6a/Unamgenomicsdeepdescriptionbacillus1.png' height="200" />
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<img src='https://static.igem.org/mediawiki/2012/a/a8/UnamgenomicsoANDcadmio09_16_2012.jpg' height="300"/>
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<div class='captionaqua'>
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<p class='captionInside'>1.AmyE 5’ 97 S/P. <br />
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2.
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3.AmyE 5’ 98 S/P. <br />
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4.
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5.1 kb ladder. <br />
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6.AmyE 5’ 99 S/P. <br />
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7.AmyE 5’ 99 S/P. <br />
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8.E0040 B0014 X/P. <br />
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9.
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<img src='https://static.igem.org/mediawiki/2012/8/89/UnamgenomicsoANDcadmio09_16_2012_2.jpg' width="300"/>
<div class='captiongray'>
<div class='captiongray'>
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<p class='captionInside'>2011 Gyanendra P. Dubey, Sigal Ben-Yehuda. Intercellular Nanotubes Mediate Bacterial Communication. Cell, 2011; 144 (4): 590 DOI:10.1016/j.cell.2011.01.015<br /></p>
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<p class='captionInside'>1.E0040 B0014 X/P to extract. <br />
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2.1 kb ladder. <br />
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3.Lysis. <br />
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4.-19. Amy 5’ 99/97/P4CIE1010B0014 lysis. <br />
</div>
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</li>
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<img src='https://static.igem.org/mediawiki/2012/c/c9/Unamgenomicsdeepdescriptionbacillus2.jpg' height="200" />
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<img src='https://static.igem.org/mediawiki/2012/b/b0/UnamgenomicsoANDcadmio09_16_2012_3.jpg' height="300"/>
<div class='captionnaranja'>
<div class='captionnaranja'>
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<p class='captionInside'>2011 Gyanendra P. Dubey, Sigal Ben-Yehuda. Intercellular Nanotubes Mediate Bacterial Communication. Cell, 2011; 144 (4): 590 DOI:10.1016/j.cell.2011.01.015<br /></p>
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<p class='captionInside'>1.LasR PCR. <br />
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2.LasR PCR. <br />
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3.LasR PCR. <br />
</div>
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<div class='clear'></div>
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<img src='https://static.igem.org/mediawiki/2012/c/c1/UnamgenomicsoANDcadmio09_16_2012_4.jpg' height="300"/>
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<div class='captionrojo'>
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<p class='captionInside'>1.1 kb ladder. <br />
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2.Purified P4. <br />
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3.Purified LasR. <br />
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</div>
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</li>
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</ul>
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<img src='https://static.igem.org/mediawiki/2012/3/38/UnamgenomicsoANDcadmio09_16_2012_5.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>Top part is degraded.
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Bottom:
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1.99 E/P. <br />
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2.99 E/P. <br />
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3.CI 02 E/P. <br />
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4.CI 02 E/P. <br />
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</div>
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</li>
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</ul>
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<h2>09/16/12</h2><br />
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<img src='https://static.igem.org/mediawiki/2012/d/d6/UnamgenomicsoANDcadmio09_18_2012.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>A3/Pveg lysis. <br /><br />
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<img src='https://static.igem.org/mediawiki/2012/8/8f/UnamgenomicsoANDcadmio09_18_2012_2.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>1.-12. psCIC3 omega lysis. <br />
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13. 1 kb ladder. <br />
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14. psBIc3-GusA. <br />
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<br />
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<img src='https://static.igem.org/mediawiki/2012/e/ec/UnamgenomicsoANDcadmio09_18_2012_3.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>
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<img src='https://static.igem.org/mediawiki/2012/f/fa/UnamgenomicsoANDcadmio09_18_2012_4.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>Digested A3 and pVeg with E/P. <br />
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<img src='https://static.igem.org/mediawiki/2012/9/94/UnamgenomicsoANDcadmio09_18_2012_5.jpg' height="300"/>
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<div class='captionverde'>
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<p class='captionInside'>1.ArsR-CzrA 97 E/P. <br />
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2.ArsR-CzrA 98 E/P. <br />
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3.ArsR-CzrA 99 E/P. <br />
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4.02-CI E/P. <br />
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5.PCR P4 purified. <br />
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</div>
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</li>
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</ul>
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<h2>09/18/12</h2><br />
}}
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Latest revision as of 06:47, 26 October 2012


UNAM-Genomics_Mexico

= MAY =

  • 1.ArsR-CzrA 97 E/P.
    2.ArsR-CzrA 98 E/P.
    3.ArsR-CzrA 99 E/P.
    4.CI 02 E/P.
    5.P4 PCR.



  • We extracted the correct band by kit
    1.ArsR-CzrA 97 E/P.
    2.ArsR-CzrA 98 E/P.
    3.ArsR-CzrA 99 E/P.
    4.CI 02 E/P.
    5.P4 PCR.



  • 1. 1 kb ladder.
    2.00179 Lasr lysis
    . 3.00179 LasR.
    4.00179 Lasr (-).
    5.LasR 00179 new primers.
    6.LasR 00179 new primers.
    7.LasR 00179 new primers (-).



  • 1.1 kb ladder.
    3.ArsR-CzrA 97 E/P.
    5.ArsR-CzrA 98 E/P.
    7.ArsR-CzrA 99 E/P.
    9.CI 02 E/P.
    11.AmyE 5’ ArsR-CzrA 97 E/S.
    13.AmyE 5’ ArsR-CzrA 98 E/S.
    15.AmyE 5’ ArsR-CzrA 99 E/S.



  • 1.ArsR-CzrA 97 E/P.
    2.ArsR-CzRA 98 E/P.
    3.1 kb ladder.
    4.ArsR-CzRA 99 E/P.
    5.CI 02 E/P.
    6.AmyE 5’ 97 E/S.
    7.AmyE 5’ 98 E/S.
    8.1 kb ladder.
    9.AmyE 5’ 99 E/S.
    10.LasR PCR.



  • 1.ArsR-CzrA 97 E/P.
    2.ArsR-CzRA 98 E/P.
    3.1 kb ladder.
    4.ArsR-CzRA 99 E/P.
    5.CI 02 E/P.
    6.AmyE 5’ 97 E/S.
    7.AmyE 5’ 98 E/S.
    8.1 kb ladder.
    9.AmyE 5’ 99 E/S.
    10.LasR PCR.



Contents

09/14/12


We extracted the correct band by kit GEL EXTRACTION PROTOCOL.

>We transformed ligation psB1c3+ArsR-CzrA 97/98/99/CI in competent DH5α cells from the pBad/pXyl AND team and in our own DH5α competent cells TRANSFORMATION PROTOCOL.















09/15/12


>We checked our transformations an did liquid cultures and passed the colonies to new plates LIQUID CULTURE PROTOCOL.
>We digested E0040+B0014 DIGESTIONS PROTOCOL.

>We ligated 97/98/99 psBIC3 LIGATION PROTOCOL.

  • 1.AmyE 5’ 97 S/P.
    2. 3.AmyE 5’ 98 S/P.
    4. 5.1 kb ladder.
    6.AmyE 5’ 99 S/P.
    7.AmyE 5’ 99 S/P.
    8.E0040 B0014 X/P.
    9.



  • 1.E0040 B0014 X/P to extract.
    2.1 kb ladder.
    3.Lysis.
    4.-19. Amy 5’ 99/97/P4CIE1010B0014 lysis.



  • 1.LasR PCR.
    2.LasR PCR.
    3.LasR PCR.



  • 1.1 kb ladder.
    2.Purified P4.
    3.Purified LasR.



  • Top part is degraded. Bottom: 1.99 E/P.
    2.99 E/P.
    3.CI 02 E/P.
    4.CI 02 E/P.



09/16/12



















  • A3/Pveg lysis.



  • 1.-12. psCIC3 omega lysis.
    13. 1 kb ladder.
    14. psBIc3-GusA.





  • Digested A3 and pVeg with E/P.




  • 1.ArsR-CzrA 97 E/P.
    2.ArsR-CzrA 98 E/P.
    3.ArsR-CzrA 99 E/P.
    4.02-CI E/P.
    5.PCR P4 purified.



09/18/12


Retrieved from "http://2012.igem.org/Team:UNAM_Genomics_Mexico/prueba"