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Team:UNAM Genomics Mexico/prueba
From 2012.igem.org
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<p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | <p class='captionInside'>1.ArsR-CzrA 97 E/P. <br /> | ||
Revision as of 22:51, 18 October 2012
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1.ArsR-CzrA 97 E/P.
2.ArsR-CzrA 98 E/P.
3.ArsR-CzrA 99 E/P.
4.CI 02 E/P.
5.P4 PCR.
05/29/2012
Our group is in charge of building part of the “and” construction. We started analyzing if the plasmid we have with P4 actually had what we needed.
The plasmid PRMn25 contains the protein P4. It has Amp100 resistance and comes in Escherichia coli NFI. Cells were lysed to make sure the plasmid was present in these cells (5000bp). We ran a gel with 3 lysis, a sample from the plasmid PFRC54 (A3 promoter) and a sample of total DNA from the strain from which PFRC54 was obtained.
