Team:UNAM Genomics Mexico/Parts

From 2012.igem.org

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<table cellspacing="1" cellpadding="0">
 
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  <tr>
 
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    <td width="209">Spectino    [ng/microliter] </td>
 
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    <td width="83">T0</td>
 
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    <td width="83">T30</td>
 
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    <td width="83">T60</td>
 
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    <td width="83">T90</td>
 
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    <td width="83">T120</td>
 
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    <td width="83">T150</td>
 
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    <td width="83">T180</td>
 
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    <td width="83">T210</td>
 
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    <td width="83">T240</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">0</td>
 
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    <td align="right">0.049</td>
 
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    <td align="right">0.129</td>
 
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    <td align="right">0.274</td>
 
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    <td align="right">0.448</td>
 
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    <td align="right">0.573</td>
 
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    <td align="right">0.736</td>
 
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    <td align="right">0.967</td>
 
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    <td align="right">0.967</td>
 
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    <td align="right">1.088</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">2.6</td>
 
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    <td align="right">0.051</td>
 
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    <td align="right">0.086</td>
 
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    <td align="right">0.208</td>
 
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    <td align="right">0.384</td>
 
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    <td align="right">0.531</td>
 
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    <td align="right">0.664</td>
 
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    <td align="right">0.925</td>
 
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    <td align="right">1.002</td>
 
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    <td align="right">1.096</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3</td>
 
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    <td align="right">0.048</td>
 
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    <td align="right">0.098</td>
 
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    <td align="right">0.23</td>
 
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    <td align="right">0.421</td>
 
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    <td align="right">0.556</td>
 
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    <td align="right">0.726</td>
 
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    <td align="right">0.873</td>
 
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    <td align="right">1.003</td>
 
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    <td align="right">1.056</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3.4</td>
 
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    <td align="right">0.049</td>
 
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    <td align="right">0.102</td>
 
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    <td align="right">0.213</td>
 
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    <td align="right">0.423</td>
 
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    <td align="right">0.574</td>
 
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    <td align="right">0.718</td>
 
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    <td align="right">0.83</td>
 
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    <td align="right">0.959</td>
 
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    <td align="right">1.073</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3.6</td>
 
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    <td align="right">0.048</td>
 
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    <td align="right">0.101</td>
 
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    <td align="right">0.211</td>
 
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    <td align="right">0.397</td>
 
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    <td align="right">0.528</td>
 
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    <td align="right">0.687</td>
 
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    <td align="right">0.833</td>
 
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    <td align="right">0.963</td>
 
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    <td align="right">1.026</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3.8</td>
 
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    <td align="right">0.046</td>
 
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    <td align="right">0.101</td>
 
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    <td align="right">0.209</td>
 
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    <td align="right">0.392</td>
 
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    <td align="right">0.532</td>
 
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    <td align="right">0.671</td>
 
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    <td align="right">0.706</td>
 
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    <td align="right">0.955</td>
 
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    <td align="right">1.009</td>
 
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  </tr>
 
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</table>
 
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[[File:Unamgenomicsomegacassete1.png]]
 
 +
[[File:Unamgenomicsomegacassete1.png|400px]]
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Second Repetition <br>
 
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<table cellspacing="0" cellpadding="0">
 
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  <tr>
 
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    <td width="209">Spectino    [ng/microliter]</td>
 
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    <td width="83">T0</td>
 
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    <td width="83">T30</td>
 
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    <td width="83">T60</td>
 
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    <td width="83">T90</td>
 
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    <td width="83">T120</td>
 
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    <td width="83">T150</td>
 
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    <td width="83">T180</td>
 
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    <td width="83">T210</td>
 
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    <td width="83">T240</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">0</td>
 
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    <td align="right">0.065</td>
 
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    <td align="right">0.12</td>
 
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    <td align="right">0.261</td>
 
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    <td align="right">0.435</td>
 
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    <td align="right">0.57</td>
 
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    <td align="right">0.742</td>
 
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    <td align="right">0.939</td>
 
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    <td align="right">0.966</td>
 
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    <td align="right">1.083</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">2.6</td>
 
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    <td align="right">0.045</td>
 
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    <td align="right">0.095</td>
 
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    <td align="right">0.263</td>
 
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    <td align="right">0.408</td>
 
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    <td align="right">0.541</td>
 
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    <td align="right">0.7</td>
 
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    <td align="right">0.897</td>
 
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    <td align="right">0.988</td>
 
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    <td align="right">1.07</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3</td>
 
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    <td align="right">0.048</td>
 
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    <td align="right">0.111</td>
 
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    <td align="right">0.219</td>
 
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    <td align="right">0.428</td>
 
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    <td align="right">0.55</td>
 
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    <td align="right">0.709</td>
 
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    <td align="right">0.851</td>
 
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    <td align="right">1</td>
 
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    <td align="right">1.043</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3.4</td>
 
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    <td align="right">0.048</td>
 
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    <td align="right">0.094</td>
 
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    <td align="right">0.192</td>
 
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    <td align="right">0.371</td>
 
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    <td align="right">0.534</td>
 
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    <td align="right">0.697</td>
 
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    <td align="right">0.844</td>
 
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    <td align="right">0.989</td>
 
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    <td align="right">1.036</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3.6</td>
 
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    <td align="right">0.04</td>
 
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    <td align="right">0.101</td>
 
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    <td align="right">0.214</td>
 
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    <td align="right">0.407</td>
 
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    <td align="right">0.542</td>
 
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    <td align="right">0.679</td>
 
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    <td align="right">0.853</td>
 
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    <td align="right">0.951</td>
 
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    <td align="right">1.067</td>
 
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  </tr>
 
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  <tr>
 
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    <td align="right">3.8</td>
 
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    <td align="right">0.049</td>
 
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    <td align="right">0.102</td>
 
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    <td align="right">0.211</td>
 
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    <td align="right">0.4</td>
 
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    <td align="right">0.552</td>
 
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    <td align="right">0.691</td>
 
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    <td align="right">0.83</td>
 
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    <td align="right">1</td>
 
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    <td align="right">1.006</td>
 
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  </tr>
 
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</table>
 
 +
Second Repetition <br>
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[[File:Unamgenomicsomegacassete2.png]]
 
 +
[[File:Unamgenomicsomegacassete2.png|400px]]
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===REFERENCES===
 
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<br>
 
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[1] http://www.shigen.nig.ac.jp/ecoli/strain/cvectorDetailAction.do?cvectorId=147<br>
 
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[2] http://2012.igem.org/Team:UNAM_Genomics_Mexico/Notebook/ANDSugar <br>
 
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[3] http://www.ihop-net.org/UniPub/iHOP/bng/174343.html<br>
 
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[4] http://www.wikigenes.org/e/gene/e/1238687.html<br>
 
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[5] Pierre Prentki and Henry M. Krisch (1984). In vitro insertional mutagenesis with a selectable DNA fragment. Gene, 29:303-313<br>
 
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[6] http://www.ncbi.nlm.nih.gov/nuccore/K02163<br>
 
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[7] http://www.uniprot.org/uniprot/P0AG05<br>
 
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[8] http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene&term=1446561<br>
 
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[9] Nancye C. Clark, Ørjan Olsvik, Jana M. Swenson, Carol A. Spiegel and Fred C. Tenover(1999) Detection of a Streptomycin/Spectinomycin Adenylyltransferase Gene ( aadA) in Enterococcus faecalis. Antimicrob. Agents Chemother. 43(1):157.<br>
 
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[10] http://www.ncbi.nlm.nih.gov/nuccore/207855?report=fasta<br>
 
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[11] Pierre Prentki, Anna Bind and Andrée Epstein (1991). Plasmid vectors for selecting ISI-promoted deletions in cloned DNA: sequence analysis of the omega interposon. Gene, 103:17-23.<br>
 
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[12] http://2008.igem.org/Team:Hawaii<br>
 
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[13] http://2008.igem.org/Team:Hawaii/Construction_of_Omega_Interposon_BioBrick<br>
 
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[14] http://2012.igem.org/Team:UNAM_Genomics_Mexico<br>
 
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[15] Link con la página de descripción general del sistema o el de la parte del OR<br>
 
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[16] Dubey GP, Ben-Yehuda S. (2011) Intercellular nanotubes mediate bacterial communication. Cell.;144(4) :590-600<br>
 
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Revision as of 00:49, 26 October 2012


UNAM-Genomics_Mexico


Our Parts






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The aminoglycoside antibiotic resistance gene (aadA +)[3,4] of pHP45 was originally carried on a 1.7-kb PvuII-HindIII fragment from the R100.1 plasmid[5]. Plasmid pHP45Ω was constructed in Pierre Prentki and Henry M. Krisch, 1984 [5] as stated in NCBI[6] with this fragment. The addA sequence can be found in [7, 8 ], it is confirmed to be the one contained in pHP45Ω[9] and the complete sequence of pHP45Ω plasmid can be found in [10, 11].

Hawaii 2008 iGEM team [12] attempted before to construct a similar cassette [13] but didn’t succeed. For iGEM UNAM Genomics México 2012 project [14], the Ω Cassette was used in the design of an OR logic gate[15] using a recently described new type of communication system between Bacillus Subtilis cells called Nanotubes[16].


Unamgenomicsomegacassete1.png


Second Repetition


Unamgenomicsomegacassete2.png