Team:UIUC-Illinois/Results/Scaffold

From 2012.igem.org

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<br/><b>Fig 1.</b> 50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 68.7oC 30 sec. annealing time, 35 cycles for amplification and 10 cycles for tethering. PUF and cCFP (lanes 2 & 6) were first amplified and then used in the tethering reaction in lane 3.  
<br/><b>Fig 1.</b> 50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 68.7oC 30 sec. annealing time, 35 cycles for amplification and 10 cycles for tethering. PUF and cCFP (lanes 2 & 6) were first amplified and then used in the tethering reaction in lane 3.  
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<br/><b>Fig 2.</b>
<br/><b>Fig 2.</b>
50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in HF Buffer, 68.7oC 30 sec. annealing time, 10 cycles of PCR.  
50mL 6 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in HF Buffer, 68.7oC 30 sec. annealing time, 10 cycles of PCR.  
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<img src="http://2012.igem.org/wiki/images/2/27/IGB_2012-07-26_15hr_27min_cCFP-pETDuet-1_Digestion%2C_PUF-cCFP_tether_gradient.jpg" ></center>
<br/><b>Fig 3.</b>
<br/><b>Fig 3.</b>
50mL 10 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 1 min. annealing time, 10 cycles of PCR, half the volume of primers used (0.5 μL instead of 1 μL).
50mL 10 well 1% agarose gel with 5 μL of 10 mg/mL EtBr, 1X TAE buffer, 130V for 25 min. PCR amplifications done in GC Buffer, 1 min. annealing time, 10 cycles of PCR, half the volume of primers used (0.5 μL instead of 1 μL).
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<br/><b>Fig 4.</b>
<br/><b>Fig 4.</b>
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<img src="http://2012.igem.org/wiki/images/2/27/IGB_2012-07-26_15hr_27min_cCFP-pETDuet-1_Digestion%2C_PUF-cCFP_tether_gradient.jpg"></center>
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<br/><b>Fig 5.</b>
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<div id="scaffold3" style="display:none">
<div id="scaffold3" style="display:none">

Revision as of 22:19, 3 October 2012

Header

Scaffold

RNA Scaffold

  • RNA Scaffold Results Overview
  • RNA Scaffold Data
  • PUF Tethering Data
  • Conclusion
  • RNA Scaffold Overview


    The results covered in this section are of the experiments overviewed in the RNA Scaffold Design section.