Team:UIUC-Illinois/Project/Future/Petrobrick

From 2012.igem.org

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<center><h2>Petrobrick Overview</h2></center>
<center><h2>Petrobrick Overview</h2></center>
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<p>As a side project, we decided to characterize a previous team’s work on an existing biobrick. For that purpose, we chose to characterize the University of Washington’s Petrobrick. The Petrobrick, once transformed into E. coli, acts as a microbial alkane production pathway. Two enzymes are co-transformed to create this biobrick: Acyl-ACP Reductase (<a href="http://partsregistry.org/Part:BBa_K590032">AAR - Bba_K90032</a>) and Aldehyde De-Carbonylase (<a href="http://partsregistry.org/Part:BBa_K590031">ADC - Bba_K90031</a>). <br/><br/>AAR reduces cellular fatty acyl-ACP from bacterial fatty acid via into fatty aldehydes. ADC then removes the carbonyl group on the fatty aldehyde, resulting in an odd number alkane chain one carbon shorter than the original Acyl-ACP fatty acid. In turn, both of the enzymes convert fatty acids into an odd number alkane by means of a constitutive protein expression plasmid.</p>
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<p>As a side project, we decided to characterize a previous team’s work on an existing biobrick. For that purpose, we chose to characterize the University of Washington’s Petrobrick. The Petrobrick, once transformed into E. coli, acts as a microbial alkane production pathway. Two enzymes are co-transformed to create this biobrick: Acyl-ACP Reductase (<a href="http://partsregistry.org/Part:BBa_K590032">AAR - Bba_K90032</a>) and Aldehyde De-Carbonylase (<a href="http://partsregistry.org/Part:BBa_K590031">ADC - Bba_K90031</a>). <br/><br/>AAR reduces cellular fatty acyl-ACP from bacterial fatty acid via into fatty aldehydes. ADC then removes the carbonyl group on the fatty aldehyde, resulting in an odd number alkane chain one carbon shorter than the original Acyl-ACP fatty acid. In turn, both of the enzymes convert fatty acids into an odd number alkane by means of a constitutive protein expression plasmid.
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<a href="http://partsregistry.org/Part:BBa_K590025:Experience">Click here to read our submission</a> on the Parts Registry experience page for the characterization of the Petrobrick.
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<div id="petro0" style="display:none">
<div id="petro0" style="display:none">
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<center><h2>Conclusion</h2></center>
<center><h2>Conclusion</h2></center>
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<p>The average yield for C15 alkanes as determined by our results was 160.2 mg/L. Our maximum yield was 190.6 mg/L. The average C15 alkane yield for the UW team was 160.3 mg/L. Based on our results, we were able to successfully reproduce the results from the UW iGEM team’s work on the Petrobrick, effectively proving its function. </p>
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<p>The average yield for C15 alkanes as determined by our results was 160.2 mg/L. Our maximum yield was 190.6 mg/L. The average C15 alkane yield for the UW team was 160.3 mg/L. Based on our results, we were able to successfully reproduce the results from the UW iGEM team’s work on the Petrobrick, effectively proving its function.
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</p>
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<a href="http://partsregistry.org/Part:BBa_K590025:Experience">Click here to read our submission</a> on the Parts Registry experience page for the characterization of the Petrobrick.
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Revision as of 19:16, 30 September 2012

Header

Petrobrick

Petrobrick
Characterization

  • Overview
  • Experimental Design
  • Results
  • Conclusion
  • Petrobrick Overview


    As a side project, we decided to characterize a previous team’s work on an existing biobrick. For that purpose, we chose to characterize the University of Washington’s Petrobrick. The Petrobrick, once transformed into E. coli, acts as a microbial alkane production pathway. Two enzymes are co-transformed to create this biobrick: Acyl-ACP Reductase (AAR - Bba_K90032) and Aldehyde De-Carbonylase (ADC - Bba_K90031).

    AAR reduces cellular fatty acyl-ACP from bacterial fatty acid via into fatty aldehydes. ADC then removes the carbonyl group on the fatty aldehyde, resulting in an odd number alkane chain one carbon shorter than the original Acyl-ACP fatty acid. In turn, both of the enzymes convert fatty acids into an odd number alkane by means of a constitutive protein expression plasmid.
    Click here to read our submission on the Parts Registry experience page for the characterization of the Petrobrick.

    Retrieved from "http://2012.igem.org/Team:UIUC-Illinois/Project/Future/Petrobrick"