Team:UIUC-Illinois/Project/Future/AssemblyLine
From 2012.igem.org
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- | Earlier this year, research at the Kee-Hong Kim lab of Purdue University had preliminary evidence showing that a | + | Earlier this year, research at the Kee-Hong Kim lab of Purdue University had preliminary evidence showing that a phenolic compound, Piceatannol, had a potent ability to inhibit the development of human adipose cells. The mechanism is based around the idea that Piceatannol interacts with a preadipocyte's (immature fat cell) insulin receptors in such a way that surpresses it's growth into a mature adipose cell. Piceatannol is a metabolite of resveratrol, a compoud currently under investigation for possible anti-cancer properites. Piceatannol differs from resveratrol by one hydroxyl group on one of the aromatic rings. |
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Piceatannol is currently very costly to synthesize. On the advent of such a discovery, we felt that if we were to engineer a pathway to optimize the production of Piceatannol from cheaper substrates through the utilization of our PUF and RNA scaffold projects, we could show the versatility of our PUF toolkit working with an RNA scaffold. | Piceatannol is currently very costly to synthesize. On the advent of such a discovery, we felt that if we were to engineer a pathway to optimize the production of Piceatannol from cheaper substrates through the utilization of our PUF and RNA scaffold projects, we could show the versatility of our PUF toolkit working with an RNA scaffold. | ||
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</ul> | </ul> | ||
</p> | </p> | ||
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<div id="assembly0" style="display:none"> | <div id="assembly0" style="display:none"> | ||
<center><h2>Enzymatic Assembly Line Overview</h2></center> | <center><h2>Enzymatic Assembly Line Overview</h2></center> | ||
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<p> | <p> | ||
- | Earlier this year, research at the Kee-Hong Kim lab of Purdue University had preliminary evidence showing that a | + | Earlier this year, research at the Kee-Hong Kim lab of Purdue University had preliminary evidence showing that a phenolic compound, Piceatannol, had a potent ability to inhibit the development of human adipose cells. The mechanism is based around the idea that Piceatannol interacts with a preadipocyte's (immature fat cell) insulin receptors in such a way that surpresses it's growth into a mature adipose cell. Piceatannol is a metabolite of resveratrol, a compoud currently under investigation for possible anti-cancer properites. Piceatannol differs from resveratrol by one hydroxyl group on one of the aromatic rings. |
<br/><br/> | <br/><br/> | ||
Piceatannol is currently very costly to synthesize. On the advent of such a discovery, we felt that if we were to engineer a pathway to optimize the production of Piceatannol from cheaper substrates through the utilization of our PUF and RNA scaffold projects, we could show the versatility of our PUF toolkit working with an RNA scaffold. | Piceatannol is currently very costly to synthesize. On the advent of such a discovery, we felt that if we were to engineer a pathway to optimize the production of Piceatannol from cheaper substrates through the utilization of our PUF and RNA scaffold projects, we could show the versatility of our PUF toolkit working with an RNA scaffold. | ||
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<center><h2>Design and Theory</h2></center> | <center><h2>Design and Theory</h2></center> | ||
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- | <img src="https://static.igem.org/mediawiki/2012/b/bd/Assembly.png" width=100% > | + | <img src="https://static.igem.org/mediawiki/2012/b/bd/Assembly.png" width=100% height=100%> |
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The above is our theoretical construct involving the three aforementioned genes. | The above is our theoretical construct involving the three aforementioned genes. | ||
- | As labeled, the sequence of enzymatic activity begins at TAL, which converts the naturally present amino acid in E. Coli, Tyrosine, into p-Coumaric Acid. | + | As labeled, the sequence of enzymatic activity begins at TAL, which converts the naturally present amino acid in E. Coli, Tyrosine, into p-Coumaric Acid. |
</div> | </div> | ||
</div> | </div> |
Revision as of 05:56, 2 October 2012