Team:UIUC-Illinois/Project/Design

From 2012.igem.org

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                     <li><a name="des3" >Non-Specific Control</a></li>
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                     <li><a name="des5" >Theoretical Results</a></li>
                     <li><a name="des5" >Theoretical Results</a></li>
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<center><h2>Reliability Experiments</h2></center><br/>
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<center><h2>Experiments</h2></center><br/>
<p>In order to eliminate the possibility of confounding factors affecting our apparent results, PUF-PIN experimental results were further reinforced with tests designed to consider other factors of our constructs possibly affecting our quantitative and qualitative measurements. Along with basic experiments testing our proposed constructs we also tested several controls relative to each of the different pieces of our constructs including the following:<br/><br/>
<p>In order to eliminate the possibility of confounding factors affecting our apparent results, PUF-PIN experimental results were further reinforced with tests designed to consider other factors of our constructs possibly affecting our quantitative and qualitative measurements. Along with basic experiments testing our proposed constructs we also tested several controls relative to each of the different pieces of our constructs including the following:<br/><br/>

Revision as of 05:40, 26 October 2012

Header

Project Design

PUF Project Design

  • Overview
  • PUF+PIN Fusion
  • YFP Reporter
  • Non-Specific Control
  • Experiments
  • Theoretical Results
  • PUF Experimental Design


    In designing our project we based our quantitative tests on fluorescence measured by a plate fluorescence reader. Our constructs were created in ways that best suit providing evidence for our hypothesis of the PUF-PIN fusion protein showing endonuclease activity. Generally, our results were collected from quantifying two main PUF-PIN fusion protein types, a wild type and a mutant type, in different conditions. These two fusion proteins had recognition sites that differed by two base pairs.

    Click on the list to the left to read about each of our constructs and why we decided to do them. All expressions were done in vivo with the DH5a strain of E.Coli on pBAD30 or pPROTet.E plasmids.

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