Team:UIUC-Illinois/Notebook/Protocols

From 2012.igem.org

(Difference between revisions)
 
Line 610: Line 610:
<center><h2>mCherry Experimental Protocols</h2></center><br/><br/>
<center><h2>mCherry Experimental Protocols</h2></center><br/><br/>
1) Inoculate 2 of each of the following cultures in 5ml LB: <br/><br/>
1) Inoculate 2 of each of the following cultures in 5ml LB: <br/><br/>
-
BL21 (no antibiotic)<br/>
+
BL21 (no antibiotic)<br/><br/>
A. <br/><br/>
A. <br/><br/>
             mcherry + Wild Type Puf Binding Site in Protet plasmid & Wild Type Puf+Pin <br/>
             mcherry + Wild Type Puf Binding Site in Protet plasmid & Wild Type Puf+Pin <br/>
             (5ul amp* + 5ul cm*)<br/>
             (5ul amp* + 5ul cm*)<br/>
             mcherry + Wild Type Puf Binding Site in Protet plasmid & Mutant Puf+Pin <br/>
             mcherry + Wild Type Puf Binding Site in Protet plasmid & Mutant Puf+Pin <br/>
-
             (5ul amp + 5ul cm)<br/>
+
             (5ul amp + 5ul cm)<br/><br/>
           B.<br/><br/>
           B.<br/><br/>
             mcherry + Mutant Puf Binding Site in Protet plasmid & Wild Type Puf+Pin <br/>
             mcherry + Mutant Puf Binding Site in Protet plasmid & Wild Type Puf+Pin <br/>
             (5ul amp + 5ul cm)<br/>
             (5ul amp + 5ul cm)<br/>
mcherry + Mutant Puf Binding Site in Protet plasmid & Mutant Puf+Pin <br/>
mcherry + Mutant Puf Binding Site in Protet plasmid & Mutant Puf+Pin <br/>
-
             (5ul amp + 5ul cm)<br/>
+
             (5ul amp + 5ul cm)<br/><br/>
           C.<br/><br/>
           C.<br/><br/>
mcherry + Control Binding Site in Protet plasmid & Wild Type Puf+Pin <br/>
mcherry + Control Binding Site in Protet plasmid & Wild Type Puf+Pin <br/>

Latest revision as of 06:30, 26 October 2012

Header

Protocols

Protocol Selection

  • Bootcamp Protocols
  • Digestions
  • Gel Purification
  • Inoculation
  • Ligation
  • Making Electrocompetent E.Coli
  • Making Electrophoresis Gels
  • Making TAE Buffers
  • Miniprep
  • PCR Protocols
  • Storage of Cells
  • Subculturing Plates
  • Transformation of E.Coli
  • mCherry Experiments
  • 4CL:STS Sequencing
  • Biosynthesis of piceatannol
  • UIUC iGEM Protocols

    The standard protocols for each technique used in our project endeavors have been documented. Unless further noted all procedures are based off of those used by the lab of C. V. Rao.