"
Team:UIUC-Illinois/Notebook/MeetingNotes
From 2012.igem.org
(Difference between revisions)
| Line 76: | Line 76: | ||
<center><h2>July-August</h2></center> | <center><h2>July-August</h2></center> | ||
| - | <li><a name=" | + | <li><a name="meet28" >7/5/12</a></li> |
| - | + | <li><a name="meet29" >7/6/12</a></li> | |
| - | <li><a name=" | + | |
| - | + | ||
<li><a name="meet30" >TBD</a></li> | <li><a name="meet30" >TBD</a></li> | ||
<li><a name="meet31" >TBD</a></li> | <li><a name="meet31" >TBD</a></li> | ||
<li><a name="meet32" >TBD</a></li> | <li><a name="meet32" >TBD</a></li> | ||
| + | <li><a name="meet33" >TBD</a></li> | ||
| + | <li><a name="meet34" >TBD</a></li> | ||
</div> | </div> | ||
| Line 88: | Line 88: | ||
<div id="meetingselection5" class="meetingselection"> | <div id="meetingselection5" class="meetingselection"> | ||
<center><h2>August-Jamboree</h2></center> | <center><h2>August-Jamboree</h2></center> | ||
| - | + | <li><a name="meet35">TBD</a></li> | |
| - | + | ||
| - | <li><a name="meet35" >TBD</a></li> | + | |
<li><a name="meet36" >TBD</a></li> | <li><a name="meet36" >TBD</a></li> | ||
<li><a name="meet37" >TBD</a></li> | <li><a name="meet37" >TBD</a></li> | ||
| Line 96: | Line 94: | ||
<li><a name="meet39" >TBD</a></li> | <li><a name="meet39" >TBD</a></li> | ||
<li><a name="meet40" >TBD</a></li> | <li><a name="meet40" >TBD</a></li> | ||
| + | <li><a name="meet41" >TBD</a></li> | ||
| + | <li><a name="meet42" >TBD</a></li> | ||
</div> | </div> | ||
| Line 2,096: | Line 2,096: | ||
- Bob - got access to the server from Bhalerao. Needs to learn linux to work it (Ubuntu). Will work on human practices page with Asha. | - Bob - got access to the server from Bhalerao. Needs to learn linux to work it (Ubuntu). Will work on human practices page with Asha. | ||
- Uros - advisor’s meeting at 3 tomorrow. Everyone should try to create some sort of presenation. (Anthony won’t be able to make it). Send presenations to Uros. miniprepped and made a glycerol stock of the pETDuet-1 from Todd. Also digested the first construct for it and will ligate it tomorrow. Also talked to Todd about synthesizing the RNA scaffold. A promising option is making a ‘mini-gene’ but it takes 2-4 days just to get a quote. Emailed Dr. Pamela Silver questions about using IDT as a synthesis (they did it without G block synthesis, so probably used the mini-gene option.) If correspondence goes well, then hopefully we can get her to look at our sequence. It might be more expensive than anticipated, but we’ll see. Also working with Anthony on how to tether split GFP to PUF. will flip one of the PUF binding sites in order to put the split GFP closer together. | - Uros - advisor’s meeting at 3 tomorrow. Everyone should try to create some sort of presenation. (Anthony won’t be able to make it). Send presenations to Uros. miniprepped and made a glycerol stock of the pETDuet-1 from Todd. Also digested the first construct for it and will ligate it tomorrow. Also talked to Todd about synthesizing the RNA scaffold. A promising option is making a ‘mini-gene’ but it takes 2-4 days just to get a quote. Emailed Dr. Pamela Silver questions about using IDT as a synthesis (they did it without G block synthesis, so probably used the mini-gene option.) If correspondence goes well, then hopefully we can get her to look at our sequence. It might be more expensive than anticipated, but we’ll see. Also working with Anthony on how to tether split GFP to PUF. will flip one of the PUF binding sites in order to put the split GFP closer together. | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
</textarea> | </textarea> | ||
| Line 2,106: | Line 2,101: | ||
<div id="meet28" style="display:none"> | <div id="meet28" style="display:none"> | ||
<textarea readonly rows="30" wrap="soft"> | <textarea readonly rows="30" wrap="soft"> | ||
| + | |||
| + | Thursday, 7/5/12 | ||
| + | - Adi, corporate/entrepreneurship - Been working with Alex on the entrepreneurship wiki, went to Chicago to do design the website with Alex. At this point have the entire structure of the wiki, but are hosting it on a different domain so that the code can get perfected and then uploaded to the iGEM wiki. So far, it looks so good. The iGEM entrepreneurship wiki should be up in around 2 weeks. Once there is data it will get uploaded and the entrepreneurship code can get transferred to the normal wiki if necessary. Also, obtain $250 from the Roy J. Carver Biotechnology Center, Courtney has given the approval to use this to buy enzymes. SARSTEDT is also going to sponsor us, but they have not specified an amount yet. | ||
| + | - Anthony, PUF biobricking - looked at gel of PSB1C3 run by Asha. The bands were not correct, so digested more. Each had 2 bands and none of them were the right size. Will use linearized plasmid next. Made LB and autoclaved more 1.5 centrifuge tubes. | ||
| + | - Asha, PUF - still waiting on WT PUF/ pBAD primers, so in the meantime I made 11 tubes of DH5a cells. And plated YFP constructs from glycerol stock to do colony PCR again. | ||
</textarea> | </textarea> | ||
| Line 2,111: | Line 2,111: | ||
<div id="meet29" style="display:none"> | <div id="meet29" style="display:none"> | ||
<textarea readonly rows="30" wrap="soft"> | <textarea readonly rows="30" wrap="soft"> | ||
| + | |||
| + | Friday, 7/6/12 | ||
| + | - Adi, Entrepreneurship - same as yesterday. entrepreneurship wiki, money from Roy J. Carver (for sequencing), some sort of sponsorship from sarstedt. DWR and Fisher haven’t really responded, so Courtney will contact them personally to try to get a response. Will contact Ginkgo Bioworks next. Will also set up times outside of advisor’s meetings to meet with Joe Bradley. | ||
| + | - Anthony, PUF - PCRed 2 tubes of WT PUF, started 3 ligations with 3 different digestions and the one type of PSB1C3 that kind of worked. After the biobricking, will help Angela and Asha with the PUF and pBAD. | ||
| + | - Isiah, Washington characterization - Don’t have to wait for alkanes, so on Monday when Divya returns they will start the media growth along with the K12 and Petrobrick. Ideally by the end of the week alkanes should be produced and that fact means the petrobrick was successfully characterized. Need filters for 500 mL of liquid. Actually would be ideal to start media growth earlier, so will talk to Divya about that. Courtney has trained Isiah on how to order things, so when she leaves we will order things through Isiah (Courtney will still approve purchases remotely). | ||
| + | - Asha, PUF - Did colony PCR on the YFP constructs, PCRed WT PUF for pBAD, ran the gel for both and saw primer dimers for all. Must redo. Using the right primers? Reused the old YFP primers as check primers, which should work but might not. Will look for the check primers. Human practices: get mike from the Art department (connected to the museum) open from 11-5 during the summer (go to the second floor and talk to them). | ||
| + | - Uros, RNA scaffolding - continued work on split GFP. Had to redo a gel so ran the digestion for 4 hours (an extra hour from last time). Did a gel on it, and will continue ligation. The split GFP should be done and sent out for sequencing on Tuesday. Next Friday is the goal for tethering the split GFP to PUF. thinking about characterizing the scaffold, but putting that off until the split GFP is done. Will need assistance with characterizing the split GFP and scaffold. Will characterize the scaffold by RTPCR (being trained currently). Will consult Pam Silver paper on how they did it (think they did microscopy, but that might not be the best option for us). | ||
| + | - Angela - We are on track according to the 2012 iGEM judging form: characterization (gold), human practices (gold), working on biobricking and characterization of PUF (silver). The sequencing and characterization of PUF will happen in parallel to save time. A deadline for finishing characterization is next Friday (7/13). That way by the end of July we will be meeting all the qualifications for the gold medal. This is a very strict deadline because we have less than 2 months left to do lab work. The regional jamboree is the first week of October, and September is completely dedicated to perfecting the poster and presentation. We are running out of time, so everyone needs to work as hard as possible. Angela, Asha, and Anthony are all working on the same PUF plan to try to finish the PUF and YFP cloning done by Monday. Then we can begin the characterization and hopefully finish that by Friday. | ||
</textarea> | </textarea> | ||
| Line 2,130: | Line 2,138: | ||
</div> | </div> | ||
<div id="meet33" style="display:none"> | <div id="meet33" style="display:none"> | ||
| + | <textarea readonly rows="30" wrap="soft"> | ||
| + | |||
| + | </textarea> | ||
| + | </div> | ||
| + | <div id="meet34" style="display:none"> | ||
<textarea readonly rows="30" wrap="soft"> | <textarea readonly rows="30" wrap="soft"> | ||
Revision as of 17:10, 9 July 2012
