Team:UIUC-Illinois/Notebook/LabNotebook
From 2012.igem.org
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<li><a name="week6puf" >Week 6</a></li> | <li><a name="week6puf" >Week 6</a></li> | ||
<li><a name="week7puf" >Week 7</a></li> | <li><a name="week7puf" >Week 7</a></li> | ||
- | <li><a name="week8puf" > | + | <li><a name="week8puf" >Further Documentation</a></li> |
- | + | ||
</div> | </div> | ||
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<div id="week8puf" style="display:none"> | <div id="week8puf" style="display:none"> | ||
<h2>Week 8 Notes</h2><br/> | <h2>Week 8 Notes</h2><br/> | ||
- | + | <table border=1> | |
+ | <tbody> | ||
+ | <tr> | ||
+ | <td>7/16/2012</td> | ||
+ | <td><br />- Ordered 16 primers for construction of pETDuet with split-CFP parts and the tethering of PUF-CFP (primer extension). <br />- 4 primers were constructed by Angela for the sequencing of MSC1 and MSC2 of pETDuet-1.<br />- Inoculated two cultures from the transformation plate (one each from the 7/7 ligation and 7/10 ligation. The culture did not grow. <br />- Reinoculated four cultures (same as before except duplicates of each with no ampicillin). <br />- Also streaked competent cells on an amp plate. Did this to see if it is problem regarding ampicillin concentration. <br />- Also started two new digestions of PUF and pBad for and construct.</td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <td>7/17/2012</td> | ||
+ | <td><br />- Ran a PCR of wtPUF and a gel to go along with it.<br />- Corrected a primer for PUF-cCFP tethering and re-submitted the order. <br />- Inoculated pETDuet-1 and K157005 as well as transformed K157006 into DH5a cells. <br />- Plated the transformation on an ampicillin plate afterword. </td> | ||
+ | </tr> | ||
+ | </tbody> | ||
+ | </table> | ||
</div> | </div> | ||
<div id="week9puf" style="display:none"> | <div id="week9puf" style="display:none"> | ||
- | <h2> | + | <h2>Further Weekly Lab Notebooks</h2><br/> |
+ | |||
+ | Please contact this team's webmaster, Bob Chen, if you would like to have a copy of our complete lab notebook by e-mail at bchen26@illinois.edu | ||
+ | |||
</div> | </div> | ||
Revision as of 02:30, 4 October 2012