Team:UIUC-Illinois/Notebook/LabNotebook
From 2012.igem.org
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<td><br />6/29/2012</td> | <td><br />6/29/2012</td> | ||
- | <td><br />- Today more M9 media was made as we were unaware that it is not suppose to be autoclaved. <br />- Did a miniprep of pSB1C3<br />- None of the eYFP constructs in DH5a grew. <br />- Conclude that neither the control eYFP of the PUF eYFP constructs were properly ligated within either pBAD or ptet. <br />- While the retransformations of BL21 grew some odd looking colonies, it is almost certain that they only contain the WT PUF plasmid and not a correct eYFP plasmid. <br />- Attempt a second ligation, used same old digest (because the bands on that were correct). <br />- Ran the ligation overnight. | + | <td><br />- Today more M9 media was made as we were unaware that it is not suppose to be autoclaved. <br />- Did a miniprep of pSB1C3<br />- None of the eYFP constructs in DH5a grew. <br />- Conclude that neither the control eYFP of the PUF eYFP constructs were properly ligated within either pBAD or ptet. <br />- While the retransformations of BL21 grew some odd looking colonies, it is almost certain that they only contain the WT PUF plasmid and not a correct eYFP plasmid. <br />- Attempt a second ligation, used same old digest (because the bands on that were correct). <br />- Ran the ligation overnight. - Inoculated the successful but weird looking and dubious BL21 plates. </td> |
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Revision as of 04:49, 3 October 2012