Team:UC Davis/Notebook/Protocols
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<li class="selected"><a href="https://2012.igem.org/Team:UC_Davis/Notebook" title="Notebook">Notebook</a> | <li class="selected"><a href="https://2012.igem.org/Team:UC_Davis/Notebook" title="Notebook">Notebook</a> | ||
<ul> | <ul> | ||
+ | <li ><a href="https://2012.igem.org/Team:UC_Davis/Notebook/Protocols">Protocols</a></li> | ||
<li ><a href="./Notebook.htm ">Week 1</a></li> | <li ><a href="./Notebook.htm ">Week 1</a></li> | ||
<li ><a href="./Notebook.htm ">Week 2</a></li> | <li ><a href="./Notebook.htm ">Week 2</a></li> |
Revision as of 03:43, 1 August 2012
Protocols
•Add 20ul sterile H2O to desired well in distribution plate.
•Incubate at room temperature for ~10 minutes.
•Transfer resuspension to microcentrifuge tube.
Materials
•Competent Cells•DNA template
•800 ul LB
•LB+Antibiotic Plates
Procedure
•Thaw competent cells on ice.•Transfer 50 uL of competent cells to chilled falcon tubes.
•Add 1 uL of template to cells (2.5 uL if dilute).
•Incubate on ice for 30 minutes.
•Heat schock in 42 °C water bath for 90 seconds.
•Immediately place back onto ice for 2 minutes.
•Add 800 uL of LB to each tube.
•Incubate at 37 °C for 1 hour.
•Place 200 uL of the transformed cells on plates containing LB and the appropriate antibiotic.
•Incubate overnight at 37 °C.