Team:UC Davis/Notebook/Protocols

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<br>•Incubate at 37 °C for 1 hour.
<br>•Incubate at 37 °C for 1 hour.
<br>•Place 200 uL of the transformed cells on plates containing LB and the appropriate antibiotic.
<br>•Place 200 uL of the transformed cells on plates containing LB and the appropriate antibiotic.
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<br>•Incubate overnight at 37 °C.
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Revision as of 23:07, 31 July 2012

Team:UC Davis - 2012.igem.org


Protocols

These are our protocols.

Registry Part Distribution Rehydration
•Add 20ul sterile H2O to desired well in distribution plate.
•Incubate at room temperature for ~10 minutes.
•Transfer resuspension to microcentrifuge tube.

Transformations

Materials

•Competent Cells
•DNA template
•800 ul LB
•LB+Antibiotic Plates

Procedure

•Thaw competent cells on ice.
•Transfer 50 uL of competent cells to chilled falcon tubes.
•Add 1 uL of template to cells (2.5 uL if dilute).
•Incubate on ice for 30 minutes.
•Heat schock in 42 °C water bath for 90 seconds.
•Immediately place back onto ice for 2 minutes.
•Add 800 uL of LB to each tube.
•Incubate at 37 °C for 1 hour.
•Place 200 uL of the transformed cells on plates containing LB and the appropriate antibiotic.
•Incubate overnight at 37 °C.

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