Team:UC Chile2/SpiderColi/Constructs

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Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012

Contents

1) First Secretion System

Working Plasmid

UC Chile-OmpA+HIV.png

This is an intermediate plasmid that we used to build the GFP Reporter Plasmid an the Protease Producer Plasmid.

GFP Reporter Plasmid

UC Chile OmpA+HIV+GFP.png

The description of the mechanism of this construct is explained in the animation below.

Protease Producer Plasmid

UC Chile-OmpA+HIV+Protease.png

This construct induces the expression of the HIV protease.

Animation describing the first secretion system

UC_Chile-Animacion-1-secrecion-1_v2.gif

Click here to open this animation from the begining in a new tab