Team:UC Chile2/SpiderColi/Constructs

From 2012.igem.org

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[[File:UC_Chile-OmpA+HIV.png|center]]
[[File:UC_Chile-OmpA+HIV.png|center]]
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<center>This is an intermediate plasmid that we used to build the GFP Reporter Plasmid an the Protease Producer Plasmid.</center>
== GFP Reporter Plasmid ==
== GFP Reporter Plasmid ==
[[File:UC_Chile_OmpA+HIV+GFP.png|center]]
[[File:UC_Chile_OmpA+HIV+GFP.png|center]]
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<center>The description of the mechanism of this construct is explained in the animation below.</center>
== Protease Producer Plasmid ==
== Protease Producer Plasmid ==
[[File:UC_Chile-OmpA+HIV+Protease.png|center]]
[[File:UC_Chile-OmpA+HIV+Protease.png|center]]
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<center>This construct induces the expression of the HIV protease.</center>
== Animation describing the first secretion system ==
== Animation describing the first secretion system ==
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<a href="2012.igem.org/wiki/images/a/a5/UC_Chile-OmpA%2BHIV.png">Click here to open this animation from the begining in a new tab </a>
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<center>
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<a href="https://static.igem.org/mediawiki/2012/3/3a/UC_Chile-Animacion-1-secrecion-1_v2(2).gif">Click here to open this animation from the begining in a new tab </a>
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Latest revision as of 03:37, 16 August 2012

Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012

Contents

1) First Secretion System

Working Plasmid

UC Chile-OmpA+HIV.png
This is an intermediate plasmid that we used to build the GFP Reporter Plasmid an the Protease Producer Plasmid.

GFP Reporter Plasmid

UC Chile OmpA+HIV+GFP.png
The description of the mechanism of this construct is explained in the animation below.

Protease Producer Plasmid

UC Chile-OmpA+HIV+Protease.png
This construct induces the expression of the HIV protease.

Animation describing the first secretion system

UC_Chile-Animacion-1-secrecion-1_v2.gif

Click here to open this animation from the begining in a new tab