Team:UC Chile2/Cyanolux/Project

From 2012.igem.org

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We have designed two constructs that have different recombination locations in the Synechocystis chromosome. We have named them according to what Utah iGEM team from 2010 proposed for [http://2010.igem.org/Construction_usu.html#Integration_Plasmid_Construction naming conventions]:
We have designed two constructs that have different recombination locations in the Synechocystis chromosome. We have named them according to what Utah iGEM team from 2010 proposed for [http://2010.igem.org/Construction_usu.html#Integration_Plasmid_Construction naming conventions]:
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<h3>pSB1C3_IntK</h3>
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<h3>pSB1C3_IntK</h3> [http://partsregistry.org/Part:BBa_K743006 BBa_K743006]
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<p>This constructs is an integrative plasmid which targets neutral recombination sites (slr0370 and sll0337). We selected this locus because it has been extensively used in the literature (CAPAZ EXAGERE?) and it shown to have no deleterious effects on Synechocystis viability. We selected Kanamycin resistance as our transformation marker. [PUT LINK TO CONSTRUCT HERE].</p>
<p>This constructs is an integrative plasmid which targets neutral recombination sites (slr0370 and sll0337). We selected this locus because it has been extensively used in the literature (CAPAZ EXAGERE?) and it shown to have no deleterious effects on Synechocystis viability. We selected Kanamycin resistance as our transformation marker. [PUT LINK TO CONSTRUCT HERE].</p>

Revision as of 13:25, 23 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012