Team:UC Chile2/Cyanolux/Constructs

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Cyanolux & Bactomithril - Pontificia Universidad Católica de Chile, iGEM 2012


C11.jpg

C1.1
Integrative plasmid designed for the expression of RFP under synechocystis psbAB promoter also with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.

C12.jpg


C1.2
This integrative plasmid is designed for the expression of RFP under synechocystis psaA2 promoter with a kanamycin resistance cassette for selection in cyanobacteria. The construct is flanked by neutral recombination sites RS1 and RS2, homologous to synechocystis chromosome.

C21.jpg

C2.1
Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter.

C22.jpg

C2.2
Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psaA2 promoter.

C31.jpg

C3
Construct designed from pPMQAK1 expression plasmid, which replicates both in E. coli and cyanobacteria and is biobrick-compatible. It codes for RFP under synechocystis psbAB promoter and cuts out the whole Ccdb toxin gene.


C32.jpg

C4.jpg

C5.jpg Hyperion construct

C6.jpg