Team:UC Chile/Results/LuxBrick

From 2012.igem.org

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(Glucose concentration in media)
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Glucose is a molecule central for the energy metabolism throughout living organisms and its the main energy source for E.coli.
Glucose is a molecule central for the energy metabolism throughout living organisms and its the main energy source for E.coli.
As reduced carbon-hydrogen bonds in glucose are oxidized through glicolisis and TCA cycle, reduced equivalents are stored in NADPH and FADH2. The majority of these electrons finally react with molecular oxygen to form water at the end of the electron transport chain (ETC), reaction that is catalized by citochrome c oxydase.
As reduced carbon-hydrogen bonds in glucose are oxidized through glicolisis and TCA cycle, reduced equivalents are stored in NADPH and FADH2. The majority of these electrons finally react with molecular oxygen to form water at the end of the electron transport chain (ETC), reaction that is catalized by citochrome c oxydase.
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This metabolic pathway is linked to the Lux light emition in two ways: the luciferase reaction consumes reducing power and in the other hand it can be considered respiration as it consumes oxygen [[#1|1]].
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This metabolic pathway is linked to the Lux light emission in two ways: the luciferase reaction consumes reducing power and in the other hand it can be considered respiration as it consumes oxygen [[#1|1]].
Our results clearly show a strong inhibition of the light emition down to non-induced levels at glucose concentrations higher than 3mM . Our hypothesis is that ETC respiration competes for oxygen with luciferase respiration, then, if glucose is added to de medium, the reduction of oxygen to water would be enhanced, making oxygen less available to luciferase.
Our results clearly show a strong inhibition of the light emition down to non-induced levels at glucose concentrations higher than 3mM . Our hypothesis is that ETC respiration competes for oxygen with luciferase respiration, then, if glucose is added to de medium, the reduction of oxygen to water would be enhanced, making oxygen less available to luciferase.
The fact that LuxAB light emittion is augmented by cytochrome c ocydase inhibition supports this idea [[#2|2]].
The fact that LuxAB light emittion is augmented by cytochrome c ocydase inhibition supports this idea [[#2|2]].

Revision as of 22:34, 26 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012