Team:UC Chile/Results/Growthcurve

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{{UC_Chile4}}
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<h2>Standardizing growth conditions</h2>
 
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While we managed to acquire an inoculum of Synechocystis PCC 6803, no conditions were set for working with Synechocystis PCC 6803 in our faculty. We proceeded to standardize our own growth conditions by building a custom made cyanobacterial incubator based on growth conditions described in the literature (References?)
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<h2>Growth curve for Synechocystis PCC 6803</h2>
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Here we show our growth curve for Synechocystis PCC 6803 using the conditions set in our [[Team:UC_Chile2/DIY | DIY]] shaking incubator (40uE/s/m²), 90 RPM, 30°C.
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While we managed to acquire an inoculum of Synechocystis PCC 6803, no conditions were set for working with Synechocystis PCC 6803 in our faculty. We proceeded to standardize our own growth conditions by building a custom made cyanobacterial incubator and setting the growth conditions based on what the majority of the literature specified ()
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Here we show our growth curve for Synechocystis PCC 6803 using the conditions set in our [[Team:UC_Chile2/DIY | DIY]] shaking incubator (40uE/s/m²), 90 RPM, 30°C.
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<br>
Methods for the growth curve characterization of Synechocystis PCC 6803 over [[Team:UC_Chile2/Protocols#SyneGrowth | here]].
Methods for the growth curve characterization of Synechocystis PCC 6803 over [[Team:UC_Chile2/Protocols#SyneGrowth | here]].
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Model at 600 nm : [[File:growth1curve1_uc_chile.jpg|center]]
Model at 600 nm : [[File:growth1curve1_uc_chile.jpg|center]]
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[[File: Figure for the growth curve.jpg| 500px |center]]
[[File: Figure for the growth curve.jpg| 500px |center]]
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Model at 730 nm : [[File:growth2curve1_uc_chile.jpg|center]]
Model at 730 nm : [[File:growth2curve1_uc_chile.jpg|center]]
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[[File: Figure for the growth curve222.jpg| 500px |center]]
[[File: Figure for the growth curve222.jpg| 500px |center]]
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[[File:growthdontrecall_uc_chile.jpg|center]]
[[File:growthdontrecall_uc_chile.jpg|center]]
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Exponential growth at 730 nm
Exponential growth at 730 nm
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[[File:linearodrel_uc_chile.jpg|center]]
[[File:linearodrel_uc_chile.jpg|center]]
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Where x is the OD measurement at 600 nm and y is the function of OD measurement at 730 nm.
[[File:lineargraphgrowth_uc_chile.jpg|center]]
[[File:lineargraphgrowth_uc_chile.jpg|center]]
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<a href="https://2012.igem.org/Team:UC_Chile/Results/Int_C"><img src="https://static.igem.org/mediawiki/2012/a/ab/UC_Chile-Continue_button.jpg" align="right">
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</html>
{{UC_Chilefooter}}
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Latest revision as of 01:18, 27 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012