Team:UC Chile/Protocols

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Concentrate the DNA to be transformed into Synechocystis to 1µg/µL in 10µL for each transformation (A total of 10 ug of the transformation plasmid).  
Concentrate the DNA to be transformed into Synechocystis to 1µg/µL in 10µL for each transformation (A total of 10 ug of the transformation plasmid).  
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Inoculate Synechocystis PCC 6803 into a sterile 250mL Erlenmeyer flask with 150mL* of liquid BG-11 and grow culture until you reach an OD730nm of 0.8 1.0 (That should take between 6 to 10 days dependending on the amount of initial inoculum).  
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Inoculate Synechocystis PCC 6803 into a sterile 250mL Erlenmeyer flask with 150mL* of liquid BG-11 and grow culture until you reach an OD730nm of 0.4 0.6, never above 0.8 (That should take between 6 to 10 days dependending on the amount of initial inoculum).  
* For each 50 mL of Synechocystis PCC 6803 you will have 5 individual transformations.  
* For each 50 mL of Synechocystis PCC 6803 you will have 5 individual transformations.  
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Revision as of 08:15, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012