Team:UC Chile/Protocols

From 2012.igem.org

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<div id="Reinoculation">
<div id="Reinoculation">
<h2>Reinoculation</h2>
<h2>Reinoculation</h2>
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<br />
This is a standard protocol for propagating cultures. The amount of initial innoculum will depend on when you will need a specific OD. Refer to [https://2012.igem.org/Team:UC_Chile/Results/Growthcurve growth curves] to calculate your needed inoculum through a standard C*V = constant relation.
This is a standard protocol for propagating cultures. The amount of initial innoculum will depend on when you will need a specific OD. Refer to [https://2012.igem.org/Team:UC_Chile/Results/Growthcurve growth curves] to calculate your needed inoculum through a standard C*V = constant relation.
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<br />
All work must be done under sterile conditions.
All work must be done under sterile conditions.
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<ul>
<ul>
<li>Autoclave a flask with enough volume to harbor at least twice the volume you require</li>
<li>Autoclave a flask with enough volume to harbor at least twice the volume you require</li>
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<div id="SyneGrowth">
<div id="SyneGrowth">
<h2>Methods for the characterization of Synechocystis PCC 6803 growth curve</h2>
<h2>Methods for the characterization of Synechocystis PCC 6803 growth curve</h2>
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This are the methods we used for setting up the growth curve experiment of our Synechocystis PCC 6803 which is further described [[Team:UC_Chile2/Characterization | here ]].
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These are the methods we used for setting up the growth curve experiment of our Synechocystis PCC 6803 which is further described [[Team:UC_Chile2/Characterization | here ]].
<h3>Materials</h3>
<h3>Materials</h3>

Revision as of 08:35, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012