Team:UC Chile/Cyanolux/Results short

From 2012.igem.org

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We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer.  
We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer.  
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<html><center><img src="https://static.igem.org/mediawiki/2012/6/6a/Kjasfkjhasdf.jpg" align="right" width="600"></center></html>
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While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.
While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This led us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.

Revision as of 04:01, 27 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012