Team:UC Chile/Cyanolux/Results short

From 2012.igem.org

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We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.
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(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
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[[File: UC_Chile-verification.jpg]]
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<h3>Bioluminometer</h3>
<h3>Bioluminometer</h3>
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We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer. While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This let us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.
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We measured bioluminescence by adding directly the substrate to the cells and measuring light-output in a Luminometer.  
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[[File: UC_Chile-decanalgraph.jpg]]
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While we could measure bioluminescence of the positive LuxBrick E.coli controls, no apparent bioluminescence was seen in our <i>Synechocystis</i> cells. This let us to think that the problem might be the size of the promoter, which if not long enough, would not be able to recruit necessary transcription factors for expression.
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<h3>High-sensitive camera</h3>
<h3>High-sensitive camera</h3>
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During the Latin America Jamboree, we had a chat with a couple of judges and a student from the Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.
During the Latin America Jamboree, we had a chat with a couple of judges and a student from the Universidad de los Andes, David Olarte, on how to induce Synechocystis with n-decanal.

Revision as of 08:01, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012