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Team:UC Chile/Cyanolux/Results short
From 2012.igem.org
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<h2>Constructs for Synechocystis</h2> | <h2>Constructs for Synechocystis</h2> | ||
| - | Using our plasmid backbones we proceeded to build our constructs for <i>Synechocystis</i> | + | Using our plasmid backbones we proceeded to build our constructs for <i>Synechocystis</i>: |
| + | Luciferase (LuxAB): | ||
[[File:UC_Chile-IntKfullplasmidResult.jpg| 480px | left]] | [[File:UC_Chile-IntKfullplasmidResult.jpg| 480px | left]] | ||
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| + | We tried many times (>6) to construct the substrate production (LuxCDEG) plasmid, however we were unsuccessful. We are currently designing another strategy to build the construct... | ||
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Revision as of 05:12, 26 October 2012
Contents |
Construction
Plasmids backbones
We built two integrative plasmid backbones for Synechocystis PCC. 6803.
Constructs for Synechocystis
Using our plasmid backbones we proceeded to build our constructs for Synechocystis:
Luciferase (LuxAB):
We tried many times (>6) to construct the substrate production (LuxCDEG) plasmid, however we were unsuccessful. We are currently designing another strategy to build the construct...
Characterization in Synechocystis
Transformation
We used our plasmid backbones to transform Synechocystis PCC. 6803.
Transformation with pSB1C3_IntK (BBa_K743006):
Transformation with pSB1C3_IntS (BBa_K743010):
Verification of constructs
We proceeded to verificate the integration of the constructs in Synechocystis.
(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
Bioluminescence Assays
Bioluminometer
-graph-
High-sensitive camera
During the Latin America Jamboree, we had a chat with a couple of judges and a student, David Olarte, on how to induce Synechocystis with n-decanal. David sent us some work he had done on luminescence assays in cyanobacteria. Following his methods finally we were able to see light emition.
Experimental Highlights
