Team:UC Chile/Cyanolux/Results short

From 2012.igem.org

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<h1>Characterization in Synechocystis</h1>
<h1>Characterization in Synechocystis</h1>
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<h2>Transformation</h2>
<h2>Transformation</h2>
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We used our plasmid backbones to transform Synechocystis PCC. 6803, to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.
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We used our plasmid backbones to transform Synechocystis PCC. 6803 to verify that our initial design could indeed integrate into <i>Synechocystis's</i> genome.
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Transformation with pSB1C3_IntK (BBa_K743015):
Transformation with pSB1C3_IntK (BBa_K743015):
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[[File:Pta_LuxABvf.JPG| 600px| center]]
[[File:Pta_LuxABvf.JPG| 600px| center]]
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Transformation with pSB1C3_IntS (BBa_K743010):
Transformation with pSB1C3_IntS (BBa_K743010):
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[[File:Trans_IntS.JPG| 600px| center]]
[[File:Trans_IntS.JPG| 600px| center]]
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After two weeks, colonies became apparent in the transformation plates for both plasmid backbones while in negative controls (transformations with no DNA) there was complete absence of surviving colonies.
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<h2>Verification of constructs</h2>
<h2>Verification of constructs</h2>
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We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.
We proceeded to verify the integration of the constructs in <i>Synechocystis</i> by doing multiple PCRs to amplify various parts.

Revision as of 08:00, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012