Construction

Plasmids backbones

We built two integrative plasmid backbones for Synechocystis PCC. 6803.

Constructs for Synechocystis

Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)

Characterization in Synechocystis

Transformation

We used our plasmid backbones to transform Synechocystis PCC. 6803.

Transformation with pSB1C3_IntK (BBa_K743006):

Verification of constructs

We proceeded to verificate the integration of the constructs in Synechocystis.

(New image with illustration of verification from outer RS1 and RS2 - October 1X something)

Bioluminescence Assays

Bioluminometer

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High-sensitive camera

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Experimental Highlights