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Team:UC Chile/Cyanolux/Results short
From 2012.igem.org
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(Created page with "{{UC_Chile4}} <h1>Construction</h1> <h2>Plasmids backbones</h2> We built two integrative plasmid backbones for <i>Synechocystis</i> PCC. 6803. -Image of constructs- And links...") |
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<h2>Plasmids backbones</h2> | <h2>Plasmids backbones</h2> | ||
| - | We built two integrative plasmid backbones for | + | We built two integrative plasmid backbones for Synechocystis PCC. 6803. |
-Image of constructs- And links | -Image of constructs- And links | ||
| - | <h2>Constructs for | + | <h2>Constructs for Synechocystis</h2> |
Using our plasmid backbones we proceeded to build our constructs for <i>Synechocystis</i> (LuxAB & LuxCDEG) | Using our plasmid backbones we proceeded to build our constructs for <i>Synechocystis</i> (LuxAB & LuxCDEG) | ||
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- Image of full construts- | - Image of full construts- | ||
| - | <h1>Characterization in | + | <h1>Characterization in Synechocystis</h1> |
<h2>Transformation</h2> | <h2>Transformation</h2> | ||
Revision as of 23:48, 25 October 2012
Contents |
Construction
Plasmids backbones
We built two integrative plasmid backbones for Synechocystis PCC. 6803.
-Image of constructs- And links
Constructs for Synechocystis
Using our plasmid backbones we proceeded to build our constructs for Synechocystis (LuxAB & LuxCDEG)
- Image of full construts-
Characterization in Synechocystis
Transformation
We used our plasmid backbones to transform Synechocystis PCC. 6803.
-Image of Syne plates with colonies-
Verification of constructs
We proceeded to verificate the integration of the constructs in Synechocystis.
(New image with illustration of verification from outer RS1 and RS2 - October 1X something)
Bioluminescence Assays
Bioluminometer
-graph-
High-sensitive camera
-image-
Experimental Highlights
