Team:UC Chile/Cyanolux/Project short

From 2012.igem.org

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The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.
The importance of Biological context in Synthetic Biology has been largely underestimated. We have addressed this issue by centering our project on enhancing functionality of a previously characterized Biobrick, LuxBrick, by placing it in a context which allows new features.
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<h3>Bioluminescence</h3>
<h3>Bioluminescence</h3>
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In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.
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<h3>Chassis</h3>
<h3>Chassis</h3>
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Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.
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<h2>Strategy</h2>
<h2>Strategy</h2>
According to literature (CITA!), the limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).
According to literature (CITA!), the limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).
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It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.
It assumes that the metabolite's production is controlled by enzymes under the control of promoter 1 and it´s degradation by enzymes under promoter 2.
For more details please check [2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]
For more details please check [2012.igem.org/Team:UC_Chile/Cyanolux/Modelling here]
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With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.
With the relevance of context in mind, a biomimetic biolamp structure was designed that resembles the organ in which Vibrio fischeri -the bacteria from which the lux genes were biobricked- lives.
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[http://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]
[http://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp Full description of the device here]

Revision as of 04:02, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012