Team:UC Chile/Cyanolux/Project short

From 2012.igem.org

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In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.
In 2010 the Cambridge iGEM team Biobricked the LuxBrick, a collection of genes from the Lux operon that incorporate both the Luciferase and the substrate production enzymes without regulation, allowing endogenous bioluminescence on E. coli.
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<h3>Chassis</h3>
<h3>Chassis</h3>
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Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.
Coupling the endogenous circadian rhythms of this organism to the expression of the Lux genes will enable high-level functionality, through an automatically switching system that turns on bioluminescence only when needed.
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<h2>Strategy</h2>
<h2>Strategy</h2>
According to literature (CITA!), the liminting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control the light emition over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).
According to literature (CITA!), the liminting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control the light emition over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).
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In turn, the production of these enzymes can be specifically set to any desired  time of the day by fusing their CDSs to promoters controlled by the cyrcadian rythm.
In turn, the production of these enzymes can be specifically set to any desired  time of the day by fusing their CDSs to promoters controlled by the cyrcadian rythm.

Revision as of 01:16, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012