Team:UC Chile/Cyanolux/Project short

From 2012.igem.org

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<h2>Strategy</h2>
<h2>Strategy</h2>
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According to literature (CITA!), the limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).
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The limiting step for the bacterial bioluminescent reaction is the substrate (n-decanal) concentration <b>SHOULD ADD CITATION</b>, therefore, to control light emission over time we decided to control it´s abundance in the cells, which in our model is a function of the substrates generation (by Lux C, D, E and G enzymes) and consumption (by the LuxAB luciferase).
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html>
<html><center><img src="https://static.igem.org/mediawiki/2012/f/fc/Dospromotorusi.jpg" align="middle" width="850"></center></html>

Revision as of 06:34, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012