Team:UC Chile/Cyanolux/Project

From 2012.igem.org

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<p>To try our approach, we selected various promoters which could serve the purpose.  Our rational for selecting candidate promoters involved amplitude of oscillation, peak activity, hour, absence of restriction sites, predicted strength of promoter according to the role of the gene and reproducibility between experiments (based on the literature available).  We looked for promoters which would have peak expression nearby dusk hours and that were slightly out of phase to optimize production of bioluminescence according to our [http://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling mathematical models]. We prioritized promoters from genes that would be involved in central energetic metabolism as we believe that their expression would be most robust and reliable.</p>
<p>To try our approach, we selected various promoters which could serve the purpose.  Our rational for selecting candidate promoters involved amplitude of oscillation, peak activity, hour, absence of restriction sites, predicted strength of promoter according to the role of the gene and reproducibility between experiments (based on the literature available).  We looked for promoters which would have peak expression nearby dusk hours and that were slightly out of phase to optimize production of bioluminescence according to our [http://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling mathematical models]. We prioritized promoters from genes that would be involved in central energetic metabolism as we believe that their expression would be most robust and reliable.</p>
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<p>We choose the transaldolase promoter <b>(specific name here and code in Synechocystis Genome)</b> to direct the expression of the LuxAB genes and we found a couple of other promoters which filled the other requirements from above. Pcaa3  and PsigE, the former being already in Biobrick format (courtesy from the Utah team iGEM 2010).
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<p>We choose the transaldolase promoter to direct the expression of the LuxAB genes and we found a couple of other promoters which filled the other requirements from above. Pcaa3  and PsigE, the former being already in Biobrick format (courtesy from the Utah team iGEM 2010).
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Revision as of 03:59, 27 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012