Team:UC Chile/Cyanolux/Future

From 2012.igem.org

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That´s why we are designing new versions of our chosen promoters. Now we have changed the promoter construction methodology in terms of the lenght of the upstream sequence considered.
That´s why we are designing new versions of our chosen promoters. Now we have changed the promoter construction methodology in terms of the lenght of the upstream sequence considered.
Instead of considering just 150-200bp, up to 1000bp will be amplified from Synechocystis genome.
Instead of considering just 150-200bp, up to 1000bp will be amplified from Synechocystis genome.
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<h1>Transcriptional verification</h1>
<h1>Transcriptional verification</h1>
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We have allready assembled part [http://partsregistry.org/Part:BBa_K743010 psb1C3_IntS] wich has a double goal: to insert LuxCDEG genes into Synechocystis and to confere a copper suceptibility that makes this cells unable to thrive in the enviroment.
We have allready assembled part [http://partsregistry.org/Part:BBa_K743010 psb1C3_IntS] wich has a double goal: to insert LuxCDEG genes into Synechocystis and to confere a copper suceptibility that makes this cells unable to thrive in the enviroment.
We are also designing primers to built our [http://2012.igem.org/File:UC_Chile-Animacion-mE-gen.gif new biosafety system ] based on mE genes and MgSO4, wich will be tested as soon as it is ready.
We are also designing primers to built our [http://2012.igem.org/File:UC_Chile-Animacion-mE-gen.gif new biosafety system ] based on mE genes and MgSO4, wich will be tested as soon as it is ready.
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<h1>Biolamp development</h1>
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When all the lux genes are inserted into Synechocystis under the promoters recomended by our [http://2012.igem.org/Team:UC_Chile/Cyanolux/Modelling modelling] we will make a new bioluminescens assay in the luminometer to test if our strain is ready to be grown in the biolamp.
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The [http://2012.igem.org/Team:UC_Chile/Cyanolux/Biolamp biolamp prototype] will be built so bacteria can be placed inside it.
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Finally we will characterize the sustainability of wt Synechocystis and our recombinant strain inside the biolamp.
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Revision as of 03:45, 27 September 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012