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Team:UC Chile/Cyanolux/Biobricks
From 2012.igem.org
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Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly. | Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly. | ||
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[[File:UC Chile-BBa_K743001.jpg | 250px ]] | [[File:UC Chile-BBa_K743001.jpg | 250px ]] | ||
<i>Synechocystis</i> PCC. 6803 Citocrome C promoter. | <i>Synechocystis</i> PCC. 6803 Citocrome C promoter. | ||
Revision as of 09:51, 26 October 2012
Contents |
Plasmid backbones
pSB1C3_IntK (BBa_K743006)
Neutral recombination plasmid backbone. Kanamycin resistance as a selectable marker for integration. EcoRI restriction site in RS2. Plasmid backbone designed for Gibson Assembly.
pSB1C3_IntS (BBa_K743010)
Suceptibility generating plasmid backbone. Spectinomycin resistance as a selectable marker for integration. mRFP1 under constitutive promoter in E.coli for negative selection of standard assembly.
Biobricks and Constructs
BBa_K743000
First recombination site of IntK plasmid backbone.
BBa_K743001
Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly.
Synechocystis PCC. 6803 Citocrome C promoter.
BBa_K743002
BBa_K743009
pSB1C3_IntK(BBa_K743006) with mRFP1 under PpsaAB promoter.
BBa_K743016
pSB1C3_IntK(BBa_K743006) with mRFP1 under PpsbA2 promoter.
BBa_K743014
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminiscent under Transaldolase promoter.
BBa_K743015
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under Transaldolase promoter.
BBa_K743018
pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under Transaldolase promoter.
