Team:UC Chile/Cyanolux/Biobricks

From 2012.igem.org

(Difference between revisions)
 
Line 8: Line 8:
[[File:UC Chile-IntKplasmidResult.jpg | 400px ]]
[[File:UC Chile-IntKplasmidResult.jpg | 400px ]]
-
<br />
 
<br />
<br />
<h2>pSB1C3_IntS (BBa_K743010)</h2>
<h2>pSB1C3_IntS (BBa_K743010)</h2>
Line 14: Line 13:
[[File:UC Chile-IntSplasmidResult.jpg | 600px ]]
[[File:UC Chile-IntSplasmidResult.jpg | 600px ]]
-
<br />
 
<br />
<br />
<h1>Biobricks and Constructs</h1>
<h1>Biobricks and Constructs</h1>
 +
<br />
<h2>BBa_K743000</h2>
<h2>BBa_K743000</h2>
 +
<br />
First recombination site of IntK plasmid backbone.
First recombination site of IntK plasmid backbone.
Line 27: Line 27:
<h2>BBa_K743001</h2>
<h2>BBa_K743001</h2>
 +
<br />
Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly.
Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly.
Line 34: Line 35:
<h2>BBa_K743002</h2>
<h2>BBa_K743002</h2>
 +
<br />
<i>Synechocystis</i> PCC. 6803 Citocrome C promoter.
<i>Synechocystis</i> PCC. 6803 Citocrome C promoter.
Line 41: Line 43:
<h2>BBa_K743009</h2>
<h2>BBa_K743009</h2>
 +
<br />
pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsaAB promoter.
pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsaAB promoter.
Line 48: Line 51:
<h2>BBa_K743016</h2>
<h2>BBa_K743016</h2>
 +
<br />
pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsbA2 promoter.
pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsbA2 promoter.
Line 55: Line 59:
<h2>BBa_K743014</h2>
<h2>BBa_K743014</h2>
 +
<br />
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminescent under <i>Synechocystis</i> Transaldolase promoter.
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminescent under <i>Synechocystis</i> Transaldolase promoter.
[[File:UC Chile-IntKplasmidLuxABxl.jpg | 600px ]]
[[File:UC Chile-IntKplasmidLuxABxl.jpg | 600px ]]
Line 61: Line 66:
<h2>BBa_K743015</h2>
<h2>BBa_K743015</h2>
 +
<br />
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under <i>Synechocystis</i> Transaldolase promoter.
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under <i>Synechocystis</i> Transaldolase promoter.
Line 68: Line 74:
<h2>BBa_K743018</h2>
<h2>BBa_K743018</h2>
 +
<br />
pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under <i>Synechocystis</i> Transaldolase promoter.
pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under <i>Synechocystis</i> Transaldolase promoter.

Latest revision as of 09:55, 26 October 2012

Project: Luxilla - Pontificia Universidad Católica de Chile, iGEM 2012